Detailed information of TA system TA066514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 59159..59739
• Replicon: plasmid unnamed1
• Accession: NZ_CP017427
• Organism: Methylobacterium sp. XJLW

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: A3862_RS30405
• Protein ID: WP_111474441.1
• Coordinates: 59159..59485 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: A3862_RS30410
• Protein ID: WP_111474442.1
• Coordinates: 59482..59739 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3862_RS31765	54547..54705	-	159	WP_164706447.1	hypothetical protein	-
A3862_RS30385	54772..55044	-	273	WP_111474440.1	hypothetical protein	-
A3862_RS30390	55537..56005	+	469	Protein_55	hypothetical protein	-
A3862_RS30395	56111..56431	-	321	WP_205782378.1	hypothetical protein	-
A3862_RS30400	56431..58563	-	2133	WP_205782380.1	TonB-dependent receptor	-
A3862_RS30405	59159..59485	-	327	WP_111474441.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A3862_RS30410	59482..59739	-	258	WP_111474442.1	antitoxin	Antitoxin
A3862_RS30415	60022..62271	+	2250	WP_111474443.1	ParB/RepB/Spo0J family partition protein	-
A3862_RS30420	62250..62810	-	561	WP_111474444.1	MucR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..168543	168543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11876.64 Da
• Isoelectric Point: 10.3621

>T66514 WP_111474441.1 NZ_CP017427:c59485-59159 [Methylobacterium sp. XJLW]
MKRGDVYMVDLEPTQGREQRGHRPVVIVSPDEFNRATNLPIVAPITNGGDFARRIGFAVSLTGTRTTGVVRCDQPRVLDL
NARNGRRVESLPAAIMDDVLARMATLFA

Nucleotide

• Length: 327 bp

>T66514 NZ_CP017427:c59485-59159 [Methylobacterium sp. XJLW]
ATGAAGCGCGGTGATGTCTACATGGTCGACCTTGAACCGACCCAAGGTCGGGAACAGCGCGGGCACCGGCCGGTCGTCAT
CGTCTCGCCCGACGAGTTCAACCGTGCGACCAACCTGCCGATCGTTGCTCCGATCACGAATGGCGGCGACTTCGCCCGCC
GGATCGGCTTTGCCGTGTCGCTGACCGGGACCAGGACCACAGGGGTCGTGCGCTGCGATCAACCGCGTGTTCTCGACCTG
AATGCACGCAACGGCCGGAGAGTCGAGAGCTTACCCGCAGCGATCATGGACGACGTGTTGGCGAGGATGGCAACACTCTT
CGCGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9156.50 Da
• Isoelectric Point: 4.6816

>AT66514 WP_111474442.1 NZ_CP017427:c59739-59482 [Methylobacterium sp. XJLW]
MHTTNLRKVGGSIMLAVPPALLDVLHLTAGAKVGLTVDNGRLVVEPQARPRYTMAELLAASDYSQPQPPEEREWVDAPAI
GDEPL

Nucleotide

• Length: 258 bp

>AT66514 NZ_CP017427:c59739-59482 [Methylobacterium sp. XJLW]
ATGCATACCACCAACCTCCGCAAGGTCGGCGGATCGATCATGTTGGCCGTGCCGCCGGCGCTGCTCGACGTTCTGCACCT
GACGGCTGGCGCCAAGGTTGGCCTCACCGTCGACAATGGCCGGCTGGTGGTCGAGCCGCAGGCCCGGCCTCGCTACACGA
TGGCTGAGTTGCTGGCCGCCTCCGACTACTCTCAGCCACAGCCACCCGAGGAGCGCGAATGGGTGGATGCCCCGGCCATC
GGCGATGAGCCGCTATGA