Detailed information of TA system TA066511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 4782187..4783643
• Replicon: chromosome
• Accession: NZ_CP017420
• Organism: Delftia tsuruhatensis strain CM13

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A1D8J579
• Locus tag: BI380_RS22140
• Protein ID: WP_046239356.1
• Coordinates: 4782187..4783032 (-)
• Length: 282 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: BI380_RS22145
• Protein ID: WP_016447321.1
• Coordinates: 4783029..4783643 (-)
• Length: 205 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI380_RS22115	4777540..4778703	+	1164	WP_046238979.1	ABC transporter substrate-binding protein	-
BI380_RS22120	4778754..4779626	+	873	WP_013799989.1	branched-chain amino acid ABC transporter permease	-
BI380_RS22125	4779630..4780697	+	1068	WP_046238980.1	branched-chain amino acid ABC transporter permease	-
BI380_RS22130	4780690..4781448	+	759	WP_043823391.1	ABC transporter ATP-binding protein	-
BI380_RS22135	4781453..4782154	+	702	WP_017405695.1	ABC transporter ATP-binding protein	-
BI380_RS22140	4782187..4783032	-	846	WP_046239356.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
BI380_RS22145	4783029..4783643	-	615	WP_016447321.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
BI380_RS22150	4783751..4784224	-	474	WP_012202024.1	hotdog fold thioesterase	-
BI380_RS22155	4784355..4785869	-	1515	WP_012202025.1	acetyl-CoA hydrolase/transferase family protein	-
BI380_RS22160	4786077..4787384	-	1308	WP_046239355.1	NCS2 family nucleobase:cation symporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-258)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-57)

Sequences

Toxin

Protein

• Length: 282 a.a.
• Molecular weight: 31079.80 Da
• Isoelectric Point: 8.1191

>T66511 WP_046239356.1 NZ_CP017420:c4783032-4782187 [Delftia tsuruhatensis]
MSRDIAASIRARLKQCADARRQDFNLTLTHYGLERLLYRLSISPHADSYLLKGALLFAIWYEQPHRPTRDADLLGFGLDD
VDSAVSAFREICRIAVDDGIAFDAASVRGTAIRKEAGYGGVRIDLRATLDGARITLQVDIGFGDAVTPSPQAIRYPVLLD
GLPAPQLRSYPKYTVVAEKLHAICLLGMANTRMKDYFDLWVLMTEGTLDPAELRRAMKATFERRQLALPAGLPAGLSDGF
SSDAAKQAQWAAFLKKNRLEALELAPVVARLREEFQQCGIV

Nucleotide

• Length: 846 bp

>T66511 NZ_CP017420:c4783032-4782187 [Delftia tsuruhatensis]
ATGAGCCGCGATATCGCAGCCTCGATCCGTGCACGTTTGAAGCAATGCGCGGACGCCCGCCGGCAGGACTTCAACCTCAC
GCTCACCCACTATGGCCTGGAGCGCCTGCTGTACCGCCTGTCCATCTCGCCCCATGCGGACAGCTATCTGCTCAAGGGCG
CACTGCTGTTTGCCATCTGGTACGAGCAACCGCACAGACCGACCCGCGACGCCGATCTGCTGGGCTTTGGCCTGGACGAC
GTTGACTCGGCGGTGAGCGCTTTCCGCGAGATCTGCCGGATTGCAGTGGACGACGGCATTGCATTCGATGCCGCGTCGGT
GCGTGGAACCGCCATCCGCAAGGAAGCCGGCTATGGCGGAGTACGGATTGACCTGCGCGCCACGCTGGACGGTGCACGCA
TCACGCTGCAGGTGGACATAGGCTTCGGCGATGCCGTGACACCATCGCCACAAGCCATCCGCTACCCCGTGCTGCTCGAC
GGCCTGCCCGCACCGCAACTGCGCAGCTACCCCAAGTACACCGTGGTGGCCGAAAAACTCCACGCGATATGCCTGCTGGG
CATGGCCAATACCCGGATGAAGGACTACTTCGATCTCTGGGTGTTGATGACCGAAGGCACGCTGGACCCTGCCGAACTGC
GGCGCGCCATGAAAGCGACCTTCGAGCGCCGCCAACTCGCGCTGCCCGCAGGCCTGCCAGCCGGATTGAGCGATGGTTTC
AGCAGCGATGCGGCCAAGCAGGCGCAGTGGGCCGCCTTCCTGAAGAAGAACCGGCTTGAAGCGCTGGAACTGGCCCCTGT
CGTGGCAAGGCTGCGAGAGGAATTCCAGCAATGCGGCATCGTCTGA

Antitoxin

Protein

• Length: 205 a.a.
• Molecular weight: 21967.59 Da
• Isoelectric Point: 9.9117

>AT66511 WP_016447321.1 NZ_CP017420:c4783643-4783029 [Delftia tsuruhatensis]
MPAPGSALEQAVLALAEQRPLLRARDLAALGLPTMALSRLVAAGRLERVARGLYSLPGRPLSEHRSLAEVALRVPQGVVC
LLSALRVHGIGTQAPFEVWMAIPHHSPTPRIDQPALRAIRMSGAALSEGVETLQIDGVGVAVFNASKTVADCFKYRNKIG
LDVALEALRDGWAQRKLTADALWHYAAIDRVSNVMRPYLESIAA

Nucleotide

• Length: 615 bp

>AT66511 NZ_CP017420:c4783643-4783029 [Delftia tsuruhatensis]
ATGCCCGCTCCTGGCTCCGCGCTCGAACAAGCCGTTCTGGCACTGGCCGAACAACGGCCGCTGCTGCGCGCACGCGACCT
TGCCGCGCTTGGCCTGCCGACCATGGCGCTGAGCCGGCTGGTCGCGGCAGGCCGCCTTGAACGCGTTGCACGCGGCCTGT
ACAGCCTGCCGGGTCGGCCGCTCAGCGAGCACCGCTCGCTGGCGGAAGTGGCCCTGCGCGTGCCGCAGGGCGTGGTCTGC
CTGCTGTCCGCGCTGCGGGTCCACGGCATAGGAACGCAGGCACCCTTCGAGGTGTGGATGGCCATTCCCCATCACTCGCC
CACGCCTCGCATAGACCAGCCTGCGTTGCGCGCCATCCGCATGTCCGGTGCGGCACTGTCCGAAGGCGTGGAAACCCTGC
AGATCGATGGCGTCGGTGTCGCCGTCTTCAACGCATCGAAGACCGTGGCCGATTGCTTCAAGTACCGCAACAAGATCGGC
CTGGATGTGGCGCTGGAAGCACTGCGCGACGGATGGGCGCAGCGCAAGCTGACGGCCGACGCGCTCTGGCACTACGCAGC
CATCGACCGCGTCAGCAATGTGATGCGCCCCTACCTTGAAAGCATCGCCGCATGA

Structures

Toxin

• AlphaFold DB: A0A1D8J579