Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 44647..45248 Replicon plasmid pRI113_1
Accession NZ_CP017407
Organism Lactiplantibacillus plantarum strain RI-113

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag BJD88_RS16180 Protein ID WP_001748110.1
Coordinates 44647..44991 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag BJD88_RS16185 Protein ID WP_003643337.1
Coordinates 44985..45248 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJD88_RS16165 39787..40611 - 825 WP_064620504.1 NEAT domain-containing protein -
BJD88_RS16170 40604..42706 - 2103 WP_076632984.1 hypothetical protein -
BJD88_RS16175 43069..44328 + 1260 WP_064620510.1 cytochrome P450 -
BJD88_RS16180 44647..44991 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BJD88_RS16185 44985..45248 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BJD88_RS16190 45334..45921 + 588 WP_076632985.1 site-specific integrase -
BJD88_RS16200 46240..47039 + 800 WP_088899809.1 IS5 family transposase -
BJD88_RS16205 46999..47376 - 378 WP_077143520.1 hypothetical protein -
BJD88_RS16215 48427..49215 + 789 WP_003645724.1 AAA family ATPase -
BJD88_RS16220 49208..49441 + 234 WP_015639781.1 hypothetical protein -
BJD88_RS17710 49546..49683 + 138 WP_015639780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..51784 51784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T66500 WP_001748110.1 NZ_CP017407:c44991-44647 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T66500 NZ_CP017407:c44991-44647 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCGCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT66500 WP_003643337.1 NZ_CP017407:c45248-44985 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT66500 NZ_CP017407:c45248-44985 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTGGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References