Detailed information of TA system TA066489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5197286..5198328
• Replicon: chromosome
• Accession: NZ_CP017353
• Organism: Pseudomonas aeruginosa strain FA-HZ1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BHE76_RS24390
• Protein ID: WP_003153636.1
• Coordinates: 5197753..5198328 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: BHE76_RS24385
• Protein ID: WP_003050245.1
• Coordinates: 5197286..5197756 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHE76_RS24350	5192678..5194096	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
BHE76_RS24355	5194086..5194997	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
BHE76_RS24360	5194994..5195686	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
BHE76_RS24365	5195683..5196081	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
BHE76_RS24370	5196093..5196452	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
BHE76_RS24375	5196469..5196702	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
BHE76_RS24380	5196699..5197082	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
BHE76_RS24385	5197286..5197756	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
BHE76_RS24390	5197753..5198328	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
BHE76_RS24395	5198346..5199260	+	915	WP_023114497.1	AAA family ATPase	-
BHE76_RS24400	5199257..5199727	+	471	WP_023114496.1	hypothetical protein	-
BHE76_RS24405	5199724..5200224	+	501	WP_003090159.1	hypothetical protein	-
BHE76_RS24410	5200224..5201126	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
BHE76_RS24415	5201165..5201890	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5122728..5244441	121713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T66489 WP_003153636.1 NZ_CP017353:5197753-5198328 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T66489 NZ_CP017353:5197753-5198328 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT66489 WP_003050245.1 NZ_CP017353:5197286-5197756 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT66489 NZ_CP017353:5197286-5197756 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68