Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 4045425..4046095 Replicon chromosome
Accession NZ_CP017353
Organism Pseudomonas aeruginosa strain FA-HZ1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag BHE76_RS18945 Protein ID WP_023114647.1
Coordinates 4045676..4046095 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag BHE76_RS18940 Protein ID WP_023114648.1
Coordinates 4045425..4045679 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHE76_RS18920 4040966..4041967 - 1002 WP_031637439.1 hypothetical protein -
BHE76_RS18925 4042052..4042276 + 225 WP_023114650.1 AlpA family phage regulatory protein -
BHE76_RS18930 4042279..4043172 + 894 WP_031637438.1 helicase RepA family protein -
BHE76_RS18935 4043153..4044130 + 978 WP_031637437.1 plasmid replication -
BHE76_RS18940 4045425..4045679 + 255 WP_023114648.1 Arc family DNA-binding protein Antitoxin
BHE76_RS18945 4045676..4046095 + 420 WP_023114647.1 type II toxin-antitoxin system VapC family toxin Toxin
BHE76_RS18950 4046251..4047021 + 771 WP_023114646.1 P-type conjugative transfer protein TrbJ -
BHE76_RS33425 4047034..4047192 + 159 WP_023114645.1 hypothetical protein -
BHE76_RS18955 4047194..4048665 + 1472 Protein_3783 P-type conjugative transfer protein TrbL -
BHE76_RS18960 4048734..4048976 + 243 WP_023114643.1 type I toxin-antitoxin system ptaRNA1 family toxin -
BHE76_RS18965 4048988..4049272 + 285 WP_023114642.1 hypothetical protein -
BHE76_RS18970 4049275..4049643 + 369 WP_023114641.1 conjugal transfer transcriptional regulator TraJ -
BHE76_RS32660 4049682..4050140 - 459 WP_023114640.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4039362..4060574 21212


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

Arc-like DNA binding domain

(9-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14925.16 Da        Isoelectric Point: 5.1890

>T66487 WP_023114647.1 NZ_CP017353:4045676-4046095 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVVETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Download         Length: 420 bp

>T66487 NZ_CP017353:4045676-4046095 [Pseudomonas aeruginosa]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAGCCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGTGGAAACCCTCTACCTGTCCAGCGTGACGCTGGCCGAGCTGCTGTTCGGCATCGGCGCGCTTCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCCCTGGACGGCTTGCTCGAGCTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCCATTATGCCGAGCTTGCGGTGAAGGCCCGCACAGCCGGAAAGGGTTTCCCAACACCCGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTTGAAGCGGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9508.87 Da        Isoelectric Point: 6.4760

>AT66487 WP_023114648.1 NZ_CP017353:4045425-4045679 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Download         Length: 255 bp

>AT66487 NZ_CP017353:4045425-4045679 [Pseudomonas aeruginosa]
ATGGCAATCCTGACAGTGCGGAATGTGCCGGACGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTGCGCGAGATTCTGGAAAGTGCAGTGAAGTCGGAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCGCGTAGGGCTGACAAACGACGACTTCGCAGTATTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References