Detailed information of TA system TA066477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1239399..1240177
• Replicon: chromosome
• Accession: NZ_CP017317
• Organism: Xanthomonas campestris pv. campestris str. CN14

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: XCCCN14_RS05355
• Protein ID: WP_040940794.1
• Coordinates: 1239399..1239890 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: XCCCN14_RS05360
• Protein ID: WP_011038219.1
• Coordinates: 1239887..1240177 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XCCCN14_RS05340	1235727..1236602	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
XCCCN14_RS05345	1237252..1237929	+	678	WP_003490678.1	response regulator transcription factor	-
XCCCN14_RS05350	1237922..1239256	+	1335	WP_040940793.1	HAMP domain-containing histidine kinase	-
XCCCN14_RS05355	1239399..1239890	-	492	WP_040940794.1	GNAT family N-acetyltransferase	Toxin
XCCCN14_RS05360	1239887..1240177	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
XCCCN14_RS05365	1240252..1240653	-	402	WP_040940795.1	type I toxin-antitoxin system SymE family toxin	-
XCCCN14_RS05375	1241185..1241808	-	624	WP_040940796.1	dephospho-CoA kinase	-
XCCCN14_RS05380	1241822..1242685	-	864	WP_040940797.1	A24 family peptidase	-
XCCCN14_RS05385	1242692..1243954	-	1263	WP_040940798.1	type II secretion system F family protein	-
XCCCN14_RS05390	1244306..1244704	+	399	WP_040940799.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (21-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17560.25 Da
• Isoelectric Point: 7.9507

>T66477 WP_040940794.1 NZ_CP017317:c1239890-1239399 [Xanthomonas campestris pv. campestris str. CN14]
MTVSAPEPLTAHHRVEPLASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGCEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T66477 NZ_CP017317:c1239890-1239399 [Xanthomonas campestris pv. campestris str. CN14]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTAGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTGCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT66477 WP_011038219.1 NZ_CP017317:c1240177-1239887 [Xanthomonas campestris pv. campestris str. CN14]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT66477 NZ_CP017317:c1240177-1239887 [Xanthomonas campestris pv. campestris str. CN14]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Structures

Antitoxin

• AlphaFold DB: Q8P668