Detailed information of TA system TA066476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 5164789..5165331
• Replicon: chromosome
• Accession: NZ_CP017316
• Organism: Streptomyces rubrolavendulae strain MJM4426

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1Y2NPN0
• Locus tag: A4G23_RS21850
• Protein ID: WP_031129655.1
• Coordinates: 5165050..5165331 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A4G23_RS21845
• Protein ID: WP_031129656.1
• Coordinates: 5164789..5165046 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4G23_RS21815	5159992..5160990	-	999	WP_140160896.1	helix-turn-helix domain-containing protein	-
A4G23_RS21820	5161045..5161848	-	804	WP_031129662.1	ABC transporter permease	-
A4G23_RS21825	5161845..5162831	-	987	WP_069978386.1	ATP-binding cassette domain-containing protein	-
A4G23_RS21830	5162981..5163163	-	183	WP_069978388.1	DUF397 domain-containing protein	-
A4G23_RS21835	5163160..5163921	-	762	WP_085921612.1	helix-turn-helix transcriptional regulator	-
A4G23_RS21840	5164229..5164768	+	540	WP_079140621.1	ATP-binding protein	-
A4G23_RS21845	5164789..5165046	+	258	WP_031129656.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A4G23_RS21850	5165050..5165331	+	282	WP_031129655.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4G23_RS21855	5165445..5165765	+	321	WP_140160894.1	ATP-binding domain-containing protein	-
A4G23_RS21860	5165889..5167124	+	1236	WP_069978392.1	cytochrome P450	-
A4G23_RS21865	5167341..5168606	-	1266	WP_031129652.1	cytochrome P450	-
A4G23_RS21870	5168603..5169220	-	618	WP_069978394.1	ATP/GTP-binding protein	-
A4G23_RS21875	5169201..5169557	-	357	WP_031129648.1	DUF742 domain-containing protein	-
A4G23_RS21880	5169554..5169973	-	420	WP_031129647.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10844.55 Da
• Isoelectric Point: 10.5709

>T66476 WP_031129655.1 NZ_CP017316:5165050-5165331 [Streptomyces rubrolavendulae]
VGYVTRFTPHAQRDMLKVPRPDALRILYRLAELQKAIDGGDTTAFDIKALRSHHARWRLRVGDYRVVYTVEDGQLIVWVL
AVGNRREVYRQIP

Nucleotide

• Length: 282 bp

>T66476 NZ_CP017316:5165050-5165331 [Streptomyces rubrolavendulae]
GTGGGGTACGTCACGCGCTTCACGCCCCACGCGCAGCGCGACATGCTCAAGGTCCCGCGCCCGGACGCCCTGCGCATCCT
GTACCGCCTCGCCGAGTTGCAGAAGGCCATCGACGGAGGCGACACCACGGCGTTCGACATCAAAGCCCTGCGGAGCCACC
ATGCCCGATGGCGGCTCAGGGTCGGTGACTACCGCGTCGTCTACACCGTCGAGGACGGCCAGTTGATCGTGTGGGTCCTG
GCCGTTGGTAACCGGCGCGAGGTCTACCGGCAGATTCCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9693.76 Da
• Isoelectric Point: 4.4247

>AT66476 WP_031129656.1 NZ_CP017316:5164789-5165046 [Streptomyces rubrolavendulae]
MAEVRSHLADVIDRARREETPTIITRRGKQEAVVIDIEEYQRLRRLAEDAEEAWLNRLADEAESEGTRGSVSLEEMAALL
RTDQG

Nucleotide

• Length: 258 bp

>AT66476 NZ_CP017316:5164789-5165046 [Streptomyces rubrolavendulae]
ATGGCCGAGGTCCGCAGTCACCTGGCCGACGTCATCGACCGTGCCCGCCGGGAGGAGACTCCGACGATCATCACCCGGCG
CGGCAAGCAGGAAGCCGTCGTGATCGACATCGAGGAGTACCAGCGGCTGCGCCGTCTCGCCGAGGACGCCGAGGAGGCAT
GGCTCAACCGGCTGGCCGACGAGGCCGAGTCGGAGGGCACGCGAGGGTCGGTCTCGCTCGAAGAGATGGCCGCCCTCCTT
CGTACCGATCAGGGCTGA

Structures

Toxin

• AlphaFold DB: A0A1Y2NPN0