Detailed information of TA system TA066475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 4281466..4281991
• Replicon: chromosome
• Accession: NZ_CP017316
• Organism: Streptomyces rubrolavendulae strain MJM4426

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A4G23_RS18330
• Protein ID: WP_069977857.1
• Coordinates: 4281466..4281729 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1D8G5V8
• Locus tag: A4G23_RS18335
• Protein ID: WP_069977861.1
• Coordinates: 4281722..4281991 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4G23_RS18310	4276649..4277374	-	726	Protein_3630	site-specific integrase	-
A4G23_RS28985	4277376..4277555	+	180	Protein_3631	tetratricopeptide repeat protein	-
A4G23_RS18315	4277557..4278093	+	537	WP_069977854.1	NUDIX domain-containing protein	-
A4G23_RS18320	4278090..4278551	+	462	WP_079140233.1	hypothetical protein	-
A4G23_RS18325	4279148..4280767	+	1620	WP_079140234.1	DUF91 domain-containing protein	-
A4G23_RS18330	4281466..4281729	-	264	WP_069977857.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4G23_RS18335	4281722..4281991	-	270	WP_069977861.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A4G23_RS18340	4282941..4283438	+	498	WP_069977863.1	hypothetical protein	-
A4G23_RS18345	4283601..4284407	+	807	WP_079140235.1	SAM-dependent methyltransferase	-
A4G23_RS18350	4284534..4285430	-	897	WP_143660437.1	helix-turn-helix domain-containing protein	-
A4G23_RS18355	4285597..4285848	-	252	WP_031128870.1	DUF397 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-58)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10011.45 Da
• Isoelectric Point: 9.5565

>T66475 WP_069977857.1 NZ_CP017316:c4281729-4281466 [Streptomyces rubrolavendulae]
VSDYRTVFRPEAQAELRKVPRDMALRILAKLAELESNPLGFNTTALVSQPERRRLRVGDYRVVYTIDNGELVVWVVHVGH
RSTVYDT

Nucleotide

• Length: 264 bp

>T66475 NZ_CP017316:c4281729-4281466 [Streptomyces rubrolavendulae]
GTGAGTGATTACCGAACCGTCTTCCGGCCAGAGGCACAAGCCGAGCTCCGGAAGGTGCCTCGCGACATGGCCCTACGCAT
CCTGGCCAAACTGGCCGAACTGGAGAGCAACCCCCTCGGCTTCAACACCACCGCGCTCGTGTCACAGCCCGAACGCCGTC
GACTGCGGGTCGGCGACTACCGGGTCGTCTACACGATCGACAACGGAGAGCTCGTAGTGTGGGTCGTCCACGTCGGGCAT
CGCTCCACCGTGTACGACACCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10280.78 Da
• Isoelectric Point: 5.2172

>AT66475 WP_069977861.1 NZ_CP017316:c4281991-4281722 [Streptomyces rubrolavendulae]
MTQPLPIESIRDVRAHLAEVVERADRDDVPTVITRRGKQVAAVVSIEVLRKYQEWEEREINRIIDERMANPAPGIPIEHI
MRETLARGE

Nucleotide

• Length: 270 bp

>AT66475 NZ_CP017316:c4281991-4281722 [Streptomyces rubrolavendulae]
ATGACGCAGCCGCTACCCATAGAGTCCATCCGCGACGTACGTGCACACCTGGCCGAGGTCGTGGAGCGCGCCGATCGAGA
CGACGTGCCCACCGTGATCACCCGACGGGGCAAGCAGGTTGCCGCCGTCGTCTCCATCGAGGTGCTGCGCAAGTACCAGG
AGTGGGAAGAGCGCGAGATCAACCGGATCATCGACGAACGCATGGCCAACCCGGCACCCGGCATCCCCATCGAGCACATC
ATGAGGGAGACGCTGGCGCGCGGTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D8G5V8