Detailed information of TA system TA066474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2281995..2282563
• Replicon: chromosome
• Accession: NZ_CP017316
• Organism: Streptomyces rubrolavendulae strain MJM4426

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1D8G0Y6
• Locus tag: A4G23_RS09560
• Protein ID: WP_069976589.1
• Coordinates: 2281995..2282366 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: A4G23_RS09565
• Protein ID: WP_031131122.1
• Coordinates: 2282366..2282563 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4G23_RS09540	2277209..2277694	+	486	WP_031131112.1	Lrp/AsnC family transcriptional regulator	-
A4G23_RS09545	2277766..2278563	-	798	WP_031131114.1	hypothetical protein	-
A4G23_RS09550	2278741..2279385	-	645	WP_031131116.1	helix-turn-helix domain-containing protein	-
A4G23_RS09555	2280036..2281820	-	1785	WP_078915563.1	DEAD/DEAH box helicase	-
A4G23_RS09560	2281995..2282366	-	372	WP_069976589.1	Fic family protein	Toxin
A4G23_RS09565	2282366..2282563	-	198	WP_031131122.1	Arc family DNA-binding protein	Antitoxin
A4G23_RS09570	2282595..2284070	-	1476	WP_069976591.1	MFS transporter	-
A4G23_RS09575	2284317..2284817	-	501	WP_031131127.1	VOC family protein	-
A4G23_RS09580	2284908..2285159	-	252	WP_069976594.1	winged helix-turn-helix domain-containing protein	-
A4G23_RS09585	2285270..2285566	-	297	WP_051839351.1	winged helix-turn-helix domain-containing protein	-
A4G23_RS09590	2285702..2286028	+	327	WP_031131133.1	hypothetical protein	-
A4G23_RS09595	2286025..2286390	+	366	WP_069976599.1	hypothetical protein	-
A4G23_RS09600	2286467..2287042	-	576	WP_159029445.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (20-80)

Antitoxin

• Arc-like DNA binding domain (5-45)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13675.61 Da
• Isoelectric Point: 5.1069

>T66474 WP_069976589.1 NZ_CP017316:c2282366-2281995 [Streptomyces rubrolavendulae]
MRYLTLPELLNLAERLGTPDVRDYGLLDSALARPRSSVFGQDAYPDVWQKAAALMESIARNHGLVDGNKPLAWYATWVFL
HVNGHPLDGAFDVDEAERFVLDVCQGALDVPKIAAQLPRFAVR

Nucleotide

• Length: 372 bp

>T66474 NZ_CP017316:c2282366-2281995 [Streptomyces rubrolavendulae]
ATGCGCTACCTCACCCTGCCCGAGCTGCTCAACCTCGCCGAGCGGCTCGGCACCCCCGACGTGCGCGACTACGGCCTTCT
GGACTCCGCGCTCGCCCGGCCCCGGTCCAGCGTCTTCGGCCAGGACGCCTACCCCGACGTCTGGCAGAAGGCCGCCGCCC
TCATGGAGTCGATCGCCCGCAACCACGGGCTCGTCGACGGCAACAAGCCCCTCGCCTGGTACGCGACCTGGGTCTTCCTG
CATGTGAACGGCCATCCCCTGGACGGGGCGTTCGACGTGGACGAGGCCGAGCGGTTCGTCCTGGACGTCTGCCAGGGCGC
CCTCGACGTGCCGAAGATCGCGGCGCAGCTCCCGCGGTTCGCGGTGCGCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7412.28 Da
• Isoelectric Point: 5.1114

>AT66474 WP_031131122.1 NZ_CP017316:c2282563-2282366 [Streptomyces rubrolavendulae]
MAGLNVRFSEEELTALRERAEAEGRSMQAFAHDAVMRSVNEHSRLFDEAAEHVLKVSEELNRRLA

Nucleotide

• Length: 198 bp

>AT66474 NZ_CP017316:c2282563-2282366 [Streptomyces rubrolavendulae]
ATGGCTGGTCTGAACGTGAGGTTCTCCGAGGAAGAGCTGACCGCGCTGCGCGAGCGCGCCGAAGCGGAGGGCCGCAGCAT
GCAGGCTTTCGCGCACGACGCCGTCATGCGGTCCGTCAACGAGCACTCGCGGCTGTTCGACGAAGCCGCCGAGCACGTCC
TCAAGGTCAGTGAGGAGCTCAACCGGAGGCTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1D8G0Y6