Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 520221..520857 Replicon chromosome
Accession NZ_CP017314
Organism Bacillus subtilis strain BS38

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BSBS38_RS02665 Protein ID WP_003156187.1
Coordinates 520507..520857 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BSBS38_RS02660 Protein ID WP_003225183.1
Coordinates 520221..520502 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSBS38_RS02640 516581..517180 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
BSBS38_RS02645 517275..517640 + 366 WP_069963989.1 holo-ACP synthase -
BSBS38_RS02650 517806..518822 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
BSBS38_RS02655 518937..520105 + 1169 Protein_474 alanine racemase -
BSBS38_RS02660 520221..520502 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BSBS38_RS02665 520507..520857 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BSBS38_RS02670 520973..521797 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BSBS38_RS02675 521802..522167 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
BSBS38_RS02680 522171..522572 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
BSBS38_RS02685 522584..523591 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
BSBS38_RS02690 523653..523982 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
BSBS38_RS02695 523979..524461 + 483 WP_003234299.1 anti-sigma B factor RsbW -
BSBS38_RS02700 524427..525215 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
BSBS38_RS02705 525215..525814 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T66472 WP_003156187.1 NZ_CP017314:520507-520857 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T66472 NZ_CP017314:520507-520857 [Bacillus subtilis]
TTGATTGTGAAACGAGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT66472 WP_003225183.1 NZ_CP017314:520221-520502 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT66472 NZ_CP017314:520221-520502 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References