Detailed information of TA system TA066465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4132084..4133126
• Replicon: chromosome
• Accession: NZ_CP017311
• Organism: Hydrogenophaga sp. PBC

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: Q5W_RS19670
• Protein ID: WP_003153636.1
• Coordinates: 4132551..4133126 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: Q5W_RS19665
• Protein ID: WP_003050245.1
• Coordinates: 4132084..4132554 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q5W_RS19630	4127467..4128897	-	1431	WP_009516218.1	TIGR03752 family integrating conjugative element protein	-
Q5W_RS19635	4128887..4129795	-	909	WP_009516219.1	TIGR03749 family integrating conjugative element protein	-
Q5W_RS19640	4129792..4130484	-	693	WP_009516220.1	TIGR03746 family integrating conjugative element protein	-
Q5W_RS19645	4130481..4130879	-	399	WP_009516221.1	TIGR03750 family conjugal transfer protein	-
Q5W_RS19650	4130891..4131250	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
Q5W_RS19655	4131267..4131500	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
Q5W_RS19660	4131497..4131880	-	384	WP_009516222.1	RAQPRD family integrative conjugative element protein	-
Q5W_RS19665	4132084..4132554	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
Q5W_RS19670	4132551..4133126	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
Q5W_RS19675	4133144..4134058	+	915	WP_003050256.1	AAA family ATPase	-
Q5W_RS19680	4134055..4134525	+	471	WP_003090160.1	hypothetical protein	-
Q5W_RS25430	4134522..4134683	+	162	Protein_3945	HORMA domain containing protein	-
Q5W_RS19685	4134742..4135434	-	693	WP_009520510.1	IS6 family transposase	-
Q5W_RS19690	4135545..4136300	+	756	WP_084463011.1	LysR family transcriptional regulator	-
Q5W_RS19695	4136392..4137474	+	1083	WP_009515030.1	TRAP transporter substrate-binding protein DctP	-
Q5W_RS19700	4137566..4137817	+	252	WP_009515028.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4113155..4134800	21645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T66465 WP_003153636.1 NZ_CP017311:4132551-4133126 [Hydrogenophaga sp. PBC]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T66465 NZ_CP017311:4132551-4133126 [Hydrogenophaga sp. PBC]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT66465 WP_003050245.1 NZ_CP017311:4132084-4132554 [Hydrogenophaga sp. PBC]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT66465 NZ_CP017311:4132084-4132554 [Hydrogenophaga sp. PBC]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68