Detailed information of TA system TA066461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3812312..3813090
• Replicon: chromosome
• Accession: NZ_CP017310
• Organism: Xanthomonas campestris pv. campestris str. CN12

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: XCCCN12_RS17460
• Protein ID: WP_011038220.1
• Coordinates: 3812599..3813090 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: XCCCN12_RS17455
• Protein ID: WP_011038219.1
• Coordinates: 3812312..3812602 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XCCCN12_RS17425	3807338..3808594	+	1257	WP_075285409.1	type II secretion system F family protein	-
XCCCN12_RS17430	3808600..3809463	+	864	WP_075285408.1	A24 family peptidase	-
XCCCN12_RS17435	3809477..3810100	+	624	WP_075285407.1	dephospho-CoA kinase	-
XCCCN12_RS17450	3811836..3812237	+	402	WP_040940795.1	type I toxin-antitoxin system SymE family toxin	-
XCCCN12_RS17455	3812312..3812602	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
XCCCN12_RS17460	3812599..3813090	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
XCCCN12_RS17465	3813233..3814567	-	1335	WP_162808569.1	HAMP domain-containing histidine kinase	-
XCCCN12_RS17470	3814560..3815237	-	678	WP_003490678.1	response regulator transcription factor	-
XCCCN12_RS17475	3815887..3816762	-	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T66461 WP_011038220.1 NZ_CP017310:3812599-3813090 [Xanthomonas campestris pv. campestris str. CN12]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T66461 NZ_CP017310:3812599-3813090 [Xanthomonas campestris pv. campestris str. CN12]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT66461 WP_011038219.1 NZ_CP017310:3812312-3812602 [Xanthomonas campestris pv. campestris str. CN12]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT66461 NZ_CP017310:3812312-3812602 [Xanthomonas campestris pv. campestris str. CN12]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668