Detailed information of TA system TA066450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2656763..2657255
• Replicon: chromosome
• Accession: NZ_CP017305
• Organism: Chlorobaculum limnaeum strain DSM 1677

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: BIU88_RS13070
• Protein ID: WP_084022412.1
• Coordinates: 2656763..2657023 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: BIU88_RS12030
• Protein ID: WP_084022413.1
• Coordinates: 2657007..2657255 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIU88_RS12005	2652126..2652437	-	312	WP_069810976.1	MTH1187 family thiamine-binding protein	-
BIU88_RS12010	2652456..2653763	-	1308	WP_069810977.1	adenylosuccinate synthase	-
BIU88_RS12015	2653775..2654353	-	579	WP_069810978.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
BIU88_RS12020	2654507..2656021	-	1515	WP_069810979.1	RNA-directed DNA polymerase	-
BIU88_RS12025	2656106..2656669	-	564	WP_069811679.1	3'-5' exonuclease	-
BIU88_RS13070	2656763..2657023	-	261	WP_084022412.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BIU88_RS12030	2657007..2657255	-	249	WP_084022413.1	ribbon-helix-helix protein, CopG family	Antitoxin
BIU88_RS12035	2657545..2658780	-	1236	WP_069810981.1	hypothetical protein	-
BIU88_RS12040	2659207..2659434	+	228	WP_069810982.1	hypothetical protein	-
BIU88_RS13585	2659510..2659665	+	156	WP_157098455.1	hypothetical protein	-
BIU88_RS12045	2659891..2661345	+	1455	WP_069811682.1	cysteine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-46)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10069.73 Da
• Isoelectric Point: 10.8457

>T66450 WP_084022412.1 NZ_CP017305:c2657023-2656763 [Chlorobaculum limnaeum]
MTWKIEFAASAEKELSRLDKAAVKRILKFLRERVAIDPRSSGKALRGDHAGLWRYRIGDYRVICEIRDETVSVLVVRVGH
RREVYR

Nucleotide

• Length: 261 bp

>T66450 NZ_CP017305:c2657023-2656763 [Chlorobaculum limnaeum]
TTGACTTGGAAGATTGAGTTTGCCGCGTCAGCAGAAAAAGAGCTCTCCAGGCTGGATAAAGCCGCTGTAAAGCGGATTCT
CAAATTCCTGCGAGAGCGCGTAGCCATCGACCCCCGCTCATCGGGCAAGGCGTTACGTGGCGATCATGCCGGACTCTGGC
GGTATCGAATAGGGGATTATCGGGTGATTTGCGAAATCCGCGATGAGACCGTTTCAGTTCTTGTCGTTCGGGTCGGGCAT
CGGAGAGAGGTTTACCGGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9698.97 Da
• Isoelectric Point: 4.5359

>AT66450 WP_084022413.1 NZ_CP017305:c2657255-2657007 [Chlorobaculum limnaeum]
MEKEKADQMIGIRIPKSVGQRLDNLAKRTGRTKTWYIREAIMEHLDDLEDIYLAEQVLERVRRGEEAVSDIDEVERRLDL
ED

Nucleotide

• Length: 249 bp

>AT66450 NZ_CP017305:c2657255-2657007 [Chlorobaculum limnaeum]
ATGGAAAAGGAAAAAGCGGATCAGATGATCGGAATTCGTATTCCGAAGAGCGTAGGTCAGCGGCTGGATAACCTTGCCAA
AAGAACTGGCCGCACCAAGACCTGGTATATTCGCGAGGCGATCATGGAGCATCTCGATGATCTTGAAGATATTTATCTGG
CAGAACAGGTTCTCGAACGCGTCAGGCGGGGTGAGGAGGCGGTGTCCGATATAGACGAAGTGGAGCGTCGTCTTGACTTG
GAAGATTGA