Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 2229329..2229894 Replicon chromosome
Accession NZ_CP017305
Organism Chlorobaculum limnaeum strain DSM 1677

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D8CZS9
Locus tag BIU88_RS10070 Protein ID WP_069810638.1
Coordinates 2229550..2229894 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4Q1DHH8
Locus tag BIU88_RS10065 Protein ID WP_069810637.1
Coordinates 2229329..2229553 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BIU88_RS10040 2224513..2225262 - 750 WP_069810632.1 4-hydroxy-tetrahydrodipicolinate reductase -
BIU88_RS10045 2225308..2226192 - 885 WP_069810633.1 ParB/RepB/Spo0J family partition protein -
BIU88_RS10050 2226216..2227013 - 798 WP_069810634.1 AAA family ATPase -
BIU88_RS10055 2227121..2227750 + 630 WP_069810635.1 DNA-3-methyladenine glycosylase -
BIU88_RS10060 2227817..2229199 + 1383 WP_069810636.1 magnesium transporter -
BIU88_RS10065 2229329..2229553 + 225 WP_069810637.1 DUF433 domain-containing protein Antitoxin
BIU88_RS10070 2229550..2229894 + 345 WP_069810638.1 DUF5615 family PIN-like protein Toxin
BIU88_RS10075 2229939..2230601 - 663 WP_205632816.1 CPBP family intramembrane metalloprotease -
BIU88_RS10080 2230615..2230884 - 270 WP_069810640.1 hypothetical protein -
BIU88_RS10085 2231071..2232180 + 1110 WP_069810641.1 tRNA 2-thiouridine(34) synthase MnmA -
BIU88_RS10090 2232502..2233173 + 672 WP_069810642.1 alpha/beta fold hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(1-79)

Antitoxin

Protein of unknown function DUF433

(8-63)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12721.76 Da        Isoelectric Point: 8.0357

>T66449 WP_069810638.1 NZ_CP017305:2229550-2229894 [Chlorobaculum limnaeum]
MILWIDAHLSPSLAAWINRTYPAIQAKSLSSMNLQRAHDRTIFKAAREANAVIMSKDADFLKLVDQYGTPPQLLWITCGN
TSNARMRAVLEKSLDKAAELLLSGEPIIEISDLR

Download         Length: 345 bp

>T66449 NZ_CP017305:2229550-2229894 [Chlorobaculum limnaeum]
ATGATACTCTGGATCGATGCGCATCTTTCGCCATCATTGGCAGCGTGGATCAACCGAACATATCCGGCCATTCAGGCCAA
ATCGTTGAGCAGCATGAATTTGCAACGAGCCCATGATCGAACCATATTCAAGGCTGCCAGAGAAGCGAACGCCGTCATCA
TGAGCAAAGATGCGGACTTCCTCAAACTTGTCGATCAATACGGTACGCCACCTCAACTGCTCTGGATCACGTGTGGCAAT
ACATCGAATGCCAGAATGAGGGCGGTACTGGAAAAATCTCTCGATAAAGCTGCGGAATTATTGCTTTCGGGAGAGCCGAT
CATTGAAATCAGCGATTTGCGTTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8292.62 Da        Isoelectric Point: 4.7204

>AT66449 WP_069810637.1 NZ_CP017305:2229329-2229553 [Chlorobaculum limnaeum]
MNEWQKRISIDPNQCGGRPCIRGLRIRVIDILDLLAAGLSQDEILEELPDLEKEDILAALKYASQKLNHPVIAA

Download         Length: 225 bp

>AT66449 NZ_CP017305:2229329-2229553 [Chlorobaculum limnaeum]
ATGAACGAGTGGCAAAAGCGGATTTCCATCGATCCGAATCAATGCGGTGGACGCCCCTGCATCCGCGGATTGAGAATCAG
GGTAATCGATATTCTCGACCTTCTTGCAGCAGGCCTCAGTCAGGATGAAATCCTTGAAGAACTCCCCGATCTTGAAAAAG
AGGATATTCTGGCAGCTTTAAAATATGCCAGCCAGAAACTCAACCATCCGGTCATTGCCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8CZS9

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q1DHH8

References