Detailed information of TA system TA066447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 1478083..1478614
• Replicon: chromosome
• Accession: NZ_CP017305
• Organism: Chlorobaculum limnaeum strain DSM 1677

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1D8D5M3
• Locus tag: BIU88_RS06530
• Protein ID: WP_069809758.1
• Coordinates: 1478333..1478614 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: BIU88_RS06525
• Protein ID: WP_069809756.1
• Coordinates: 1478083..1478346 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIU88_RS06505	1473914..1474987	-	1074	WP_069809750.1	efflux RND transporter periplasmic adaptor subunit	-
BIU88_RS06510	1475126..1475854	-	729	WP_069809752.1	PhoU family transcriptional regulator	-
BIU88_RS06515	1476105..1477265	+	1161	WP_069809754.1	cation:proton antiporter	-
BIU88_RS06520	1477291..1477785	-	495	WP_069811483.1	DUF3124 domain-containing protein	-
BIU88_RS06525	1478083..1478346	+	264	WP_069809756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BIU88_RS06530	1478333..1478614	+	282	WP_069809758.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BIU88_RS06535	1478887..1479258	-	372	WP_069809760.1	hypothetical protein	-
BIU88_RS13365	1479248..1479391	-	144	WP_157098370.1	hypothetical protein	-
BIU88_RS06545	1479568..1480254	-	687	WP_069809764.1	phosphate signaling complex protein PhoU	-
BIU88_RS06550	1480273..1481130	-	858	WP_069809765.1	phosphate ABC transporter ATP-binding protein	-
BIU88_RS06555	1481136..1482482	-	1347	WP_069809767.1	phosphate ABC transporter permease PstA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (2-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10820.50 Da
• Isoelectric Point: 9.6292

>T66447 WP_069809758.1 NZ_CP017305:1478333-1478614 [Chlorobaculum limnaeum]
MRTIKFTNRFKRDYRREKSGKHGKKLDSLFEEIVNLLAEDKPLPPNAVDHSLTGTFNDCRDCHIKPDLILIYRKTDKNEL
ELIRLGSHSVLGL

Nucleotide

• Length: 282 bp

>T66447 NZ_CP017305:1478333-1478614 [Chlorobaculum limnaeum]
ATGCGGACGATTAAATTCACCAATCGCTTCAAGCGGGATTACCGAAGAGAAAAATCGGGTAAGCACGGCAAGAAACTGGA
CAGTTTGTTTGAAGAGATCGTCAACCTGCTAGCTGAGGACAAACCTTTGCCGCCAAACGCGGTCGATCACTCTTTAACGG
GAACATTCAACGATTGCCGTGACTGCCACATCAAACCTGACCTCATTCTGATCTACCGCAAAACCGACAAGAATGAACTT
GAGCTGATTCGGTTGGGTTCGCATAGCGTGTTAGGGTTATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9324.75 Da
• Isoelectric Point: 4.8279

>AT66447 WP_069809756.1 NZ_CP017305:1478083-1478346 [Chlorobaculum limnaeum]
MVENSVVRARIDAETKAEASAVLASIGLTLSDAVRLLLKRVAAEKALPFEPLVPNAETIEAIKAARRGELETVNSVKNLI
KDLNADD

Nucleotide

• Length: 264 bp

>AT66447 NZ_CP017305:1478083-1478346 [Chlorobaculum limnaeum]
ATGGTGGAAAATTCAGTTGTCAGGGCAAGGATCGACGCCGAAACCAAGGCGGAGGCATCGGCTGTGCTGGCAAGCATCGG
CCTCACGTTGTCCGACGCGGTGAGGTTGCTGCTGAAACGAGTTGCCGCAGAAAAGGCGCTTCCTTTCGAGCCGCTTGTTC
CGAATGCCGAAACAATCGAAGCGATCAAGGCCGCACGGCGTGGCGAACTTGAAACAGTAAACTCCGTTAAAAATCTGATC
AAGGATTTGAATGCGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A1D8D5M3