Detailed information of TA system TA066446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 151645..152163
• Replicon: plasmid pYLL2
• Accession: NZ_CP017304
• Organism: Rhodococcus sp. YL-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BH682_RS34785
• Protein ID: WP_003941269.1
• Coordinates: 151861..152163 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: C3JHS6
• Locus tag: BH682_RS34780
• Protein ID: WP_003941254.1
• Coordinates: 151645..151860 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH682_RS34755	147140..148378	+	1239	WP_075836617.1	hypothetical protein	-
BH682_RS34760	148339..148569	+	231	WP_075836619.1	hypothetical protein	-
BH682_RS34765	148566..149753	+	1188	WP_075836621.1	hypothetical protein	-
BH682_RS34770	149824..151125	+	1302	WP_003941272.1	AAA family ATPase	-
BH682_RS36560	151314..151502	+	189	WP_085989944.1	hypothetical protein	-
BH682_RS34780	151645..151860	+	216	WP_003941254.1	hypothetical protein	Antitoxin
BH682_RS34785	151861..152163	+	303	WP_003941269.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BH682_RS34790	152198..154153	+	1956	WP_075836685.1	hypothetical protein	-
BH682_RS34795	154153..154950	+	798	WP_075836623.1	hypothetical protein	-
BH682_RS34800	155082..156152	-	1071	WP_003941261.1	DUF1016 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..339489	339489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-94)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10456.97 Da
• Isoelectric Point: 5.0492

>T66446 WP_003941269.1 NZ_CP017304:151861-152163 [Rhodococcus sp. YL-1]
VRPIHEAQLGDLCPVLVLTRELVRPHLGTVTVAPIRSKGRGLSTELSLGEGNGVEDGSVADCDGVTTIPVSALGANRGFL
LPAQEVLLSRALRSAFDLES

Nucleotide

• Length: 303 bp

>T66446 NZ_CP017304:151861-152163 [Rhodococcus sp. YL-1]
GTGCGCCCGATCCACGAGGCGCAACTCGGAGATCTTTGTCCGGTGTTGGTCCTGACGAGGGAATTGGTTCGGCCCCACCT
GGGGACGGTCACTGTTGCCCCCATTCGTTCGAAGGGGCGGGGGTTGTCGACCGAACTGAGTTTAGGTGAAGGCAACGGCG
TCGAGGACGGATCTGTTGCCGACTGCGACGGGGTGACGACCATCCCCGTGTCGGCACTCGGTGCGAACCGAGGATTTCTC
CTGCCGGCTCAGGAAGTGTTGTTGAGCAGGGCGCTGCGATCAGCATTCGATCTCGAATCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7670.69 Da
• Isoelectric Point: 8.9167

>AT66446 WP_003941254.1 NZ_CP017304:151645-151860 [Rhodococcus sp. YL-1]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVEILSDGAGSDVNIAAAHGKKN

Nucleotide

• Length: 216 bp

>AT66446 NZ_CP017304:151645-151860 [Rhodococcus sp. YL-1]
ATGAGTACGCAGATCGCGGTACGGCTACCCGAGGACATCGTGGCGTTTCTCGACAGTGAGGTCAGCGGTGGGCGTGCGAA
AAGCCGTGCTCAAGTCGTGCTTCGAGCGTTGGAGCGTGAGCAGCGTCGACAGTTGGCGGTCCGCGACGTGGAGATCCTGT
CTGACGGCGCGGGTAGCGATGTGAATATCGCTGCCGCGCACGGGAAGAAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G9A886