Detailed information of TA system TA066445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 225303..225838
• Replicon: plasmid pYLL1
• Accession: NZ_CP017303
• Organism: Rhodococcus sp. YL-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BH682_RS33245
• Protein ID: WP_064444974.1
• Coordinates: 225303..225602 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3G9AJN8
• Locus tag: BH682_RS33250
• Protein ID: WP_042445813.1
• Coordinates: 225602..225838 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH682_RS33230	221429..222499	+	1071	WP_075835789.1	DUF1016 family protein	-
BH682_RS33235	222627..223424	-	798	WP_064688352.1	hypothetical protein	-
BH682_RS33240	223424..225274	-	1851	WP_075836257.1	hypothetical protein	-
BH682_RS33245	225303..225602	-	300	WP_064444974.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BH682_RS33250	225602..225838	-	237	WP_042445813.1	hypothetical protein	Antitoxin
BH682_RS33255	226211..226951	-	741	WP_082920770.1	TetR/AcrR family transcriptional regulator	-
BH682_RS33260	226948..227916	-	969	WP_064444976.1	DUF2637 domain-containing protein	-
BH682_RS33265	228547..228861	-	315	WP_064444977.1	hypothetical protein	-
BH682_RS33270	228858..229640	-	783	WP_029256883.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..393079	393079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10440.96 Da
• Isoelectric Point: 4.4988

>T66445 WP_064444974.1 NZ_CP017303:c225602-225303 [Rhodococcus sp. YL-1]
MRPIHEADLDTVRPVLVLTRQVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T66445 NZ_CP017303:c225602-225303 [Rhodococcus sp. YL-1]
ATGCGACCAATTCACGAAGCCGACCTCGACACAGTCCGCCCGGTCCTCGTACTCACCCGACAGGTGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGACGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGTGCCGCGAACGGAC
TATCCGCCGACGCGGTGGTCAGTTGCGATCACATCACCACCATTCCCGTCAGTGCACTCGGCAACGTCATCGGTTATCTT
CTGCCAGCACAAGAAGAATCACTGTCCATAGCCGTGCGCGCCGCATTCGATCTCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8633.62 Da
• Isoelectric Point: 6.2181

>AT66445 WP_042445813.1 NZ_CP017303:c225838-225602 [Rhodococcus sp. YL-1]
MSTQIAVRLPDEIVAFLDDEIREQRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT66445 NZ_CP017303:c225838-225602 [Rhodococcus sp. YL-1]
ATGAGCACGCAGATCGCGGTACGACTACCGGACGAGATCGTGGCATTTCTCGACGACGAAATCAGAGAACAGCGCGCCAC
CTCGCGAGCCTCGGTAGTGTTGCGCGCTCTCGAACGCGAGCGTCGCCGACAGATCGCCGCACGTGACGCGGAGATTCTCG
CGAACGCCACATCCGAACATGACCTGAGTTCTCTCGCTCACCACGCCGCCAATCCGCAGTCAACAGGCCTGAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G9AJN8