Detailed information of TA system TA066444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 274210..274745
• Replicon: plasmid pYLC1
• Accession: NZ_CP017300
• Organism: Rhodococcus sp. YL-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BH682_RS30980
• Protein ID: WP_064444974.1
• Coordinates: 274210..274509 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BH682_RS30985
• Protein ID: WP_064688354.1
• Coordinates: 274509..274745 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH682_RS30965	270336..271406	+	1071	WP_075835789.1	DUF1016 family protein	-
BH682_RS30970	271534..272331	-	798	WP_064688352.1	hypothetical protein	-
BH682_RS30975	272331..274181	-	1851	WP_075835791.1	hypothetical protein	-
BH682_RS30980	274210..274509	-	300	WP_064444974.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BH682_RS30985	274509..274745	-	237	WP_064688354.1	hypothetical protein	Antitoxin
BH682_RS36065	274810..275031	-	222	WP_157127669.1	hypothetical protein	-
BH682_RS35735	275565..275879	+	315	WP_083954780.1	hypothetical protein	-
BH682_RS31000	275900..276097	+	198	WP_075835796.1	hypothetical protein	-
BH682_RS31005	276242..277213	-	972	WP_075835799.1	DUF4192 domain-containing protein	-
BH682_RS31015	278235..278720	+	486	WP_075835804.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..423076	423076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10440.96 Da
• Isoelectric Point: 4.4988

>T66444 WP_064444974.1 NZ_CP017300:c274509-274210 [Rhodococcus sp. YL-1]
MRPIHEADLDTVRPVLVLTRQVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T66444 NZ_CP017300:c274509-274210 [Rhodococcus sp. YL-1]
ATGCGACCAATTCACGAAGCCGACCTCGACACAGTCCGCCCGGTCCTCGTACTCACCCGACAGGTGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGACGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGTGCCGCGAACGGAC
TATCCGCCGACGCGGTGGTCAGTTGCGATCACATCACCACCATTCCCGTCAGTGCACTCGGCAACGTCATCGGTTATCTT
CTGCCAGCACAAGAAGAATCACTGTCCATAGCCGTGCGCGCCGCATTCGATCTCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8642.63 Da
• Isoelectric Point: 6.4809

>AT66444 WP_064688354.1 NZ_CP017300:c274745-274509 [Rhodococcus sp. YL-1]
MSTQIAVRLPDEIVAFLDDEIREHRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT66444 NZ_CP017300:c274745-274509 [Rhodococcus sp. YL-1]
ATGAGCACGCAGATCGCGGTACGACTACCAGACGAGATCGTGGCGTTTCTCGACGACGAAATCAGGGAACACCGCGCCAC
CTCGCGGGCCTCGGTAGTGTTGCGCGCTCTCGAACGCGAGCGTCGCCGACAGATCGCCGCACGTGACGCGGAGATTCTCG
CGAACGCCACATCCGAACATGACCTGAGTTCTCTCGCTCACCACGCCGCCAATCCGCAGTCAACAGGCCTGAGCTGA