66441

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 3804323..3805365 Replicon chromosome
Accession NZ_CP017296
Organism Pseudomonas fluorescens strain Pt14

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A3E1I3F8
Locus tag BH711_RS17120 Protein ID WP_038451456.1
Coordinates 3804323..3804898 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A0W0MVK8
Locus tag BH711_RS17125 Protein ID WP_014719842.1
Coordinates 3804895..3805365 (-) Length 157 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH711_RS17090 3800381..3800710 + 330 WP_038451449.1 hypothetical protein -
BH711_RS17095 3800681..3801319 - 639 WP_020302002.1 LysE family transporter -
BH711_RS17100 3801346..3802152 - 807 WP_038451451.1 2OG-Fe(II) oxygenase family protein -
BH711_RS17105 3802378..3803070 + 693 WP_038452247.1 helix-turn-helix domain-containing protein -
BH711_RS17110 3803079..3803714 - 636 WP_038451453.1 LysE family translocator -
BH711_RS17115 3803871..3804242 + 372 WP_014719840.1 PH domain-containing protein -
BH711_RS17120 3804323..3804898 - 576 WP_038451456.1 PIN domain-containing protein Toxin
BH711_RS17125 3804895..3805365 - 471 WP_014719842.1 helix-turn-helix domain-containing protein Antitoxin
BH711_RS17130 3805560..3806714 + 1155 WP_032872684.1 S-type pyocin domain-containing protein -
BH711_RS17135 3806722..3806979 + 258 WP_014719844.1 colicin transporter -
BH711_RS17140 3807038..3807322 + 285 WP_019817493.1 barstar family protein -
BH711_RS17145 3807343..3807717 - 375 WP_019817492.1 cytochrome c family protein -
BH711_RS17150 3807748..3808179 - 432 WP_020302009.1 VOC family protein -
BH711_RS17155 3808344..3808706 + 363 WP_003194033.1 response regulator -
BH711_RS17160 3808919..3809533 + 615 WP_003194034.1 biliverdin-producing heme oxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21375.75 Da        Isoelectric Point: 4.7036

>T66441 WP_038451456.1 NZ_CP017296:c3804898-3804323 [Pseudomonas fluorescens]
MRCSPFTAVYDACVLYPAALRDLLMWLGLSGLYRARWTGQIQDEWKRNVLKNRPDLTAAQVDCTSALMDIAIPDALVTGY
EDLCSSLDLPDPDDRHVLAAAIRSKAEVIVTFNLKDFPPHILAAYDIEPMHPDDFISDLWDLDQAAVLGAAQKQRRALKK
PAMEAGSYLEMLMRQGLVQTSKAMGPYEMML

Download         Length: 576 bp

>T66441 NZ_CP017296:c3804898-3804323 [Pseudomonas fluorescens]
ATGAGGTGTTCGCCCTTTACTGCGGTGTACGATGCTTGTGTTCTCTATCCTGCGGCACTTAGGGACTTACTCATGTGGCT
TGGTTTGTCTGGTTTGTATCGAGCGCGATGGACTGGCCAGATTCAGGATGAGTGGAAGCGCAACGTACTTAAGAACCGAC
CCGATCTTACGGCGGCTCAGGTGGACTGCACGTCGGCGCTAATGGACATTGCTATACCTGACGCGTTGGTGACGGGATAT
GAGGATCTGTGTTCGAGTTTGGATTTACCAGACCCGGATGACCGGCATGTGTTGGCGGCGGCAATTCGGAGTAAAGCTGA
GGTCATCGTGACCTTCAATTTGAAAGACTTTCCACCTCATATATTGGCTGCCTATGACATTGAACCGATGCATCCGGATG
ACTTTATTTCGGATCTCTGGGATTTGGATCAGGCGGCAGTGTTGGGAGCGGCGCAGAAGCAGAGGAGGGCGTTGAAGAAG
CCCGCGATGGAGGCGGGAAGTTATCTTGAGATGTTGATGCGGCAGGGCTTGGTGCAGACTAGCAAGGCAATGGGACCCTA
TGAAATGATGCTGTGA

Antitoxin


Download         Length: 157 a.a.        Molecular weight: 17496.17 Da        Isoelectric Point: 6.1100

>AT66441 WP_014719842.1 NZ_CP017296:c3805365-3804895 [Pseudomonas fluorescens]
MTINNLPKTILPLEKEVEAAVQGQRELASLLSTKFETQRIDIFDKEDKPHRLVLPTSALRLLVDILGELALGNAVKVVPV
HAELTSQEAADLLNVSRPHLVKMLEEGAIPFTKTGRHRRVRFSDLMAFKQRRDEQSQEAMEALVQQAQELGMGYDG

Download         Length: 471 bp

>AT66441 NZ_CP017296:c3805365-3804895 [Pseudomonas fluorescens]
ATGACCATCAACAATCTTCCCAAAACGATCCTTCCGCTGGAAAAAGAAGTCGAGGCGGCAGTGCAAGGTCAGCGTGAATT
AGCGTCCCTATTGTCGACCAAATTTGAAACCCAGCGCATCGATATCTTCGACAAAGAAGACAAGCCACACAGGCTTGTCC
TACCAACCTCCGCACTGCGTCTGCTGGTCGATATTCTGGGCGAATTGGCGCTGGGCAATGCCGTCAAGGTAGTGCCGGTT
CATGCCGAGTTGACCTCGCAGGAGGCTGCCGACCTGCTTAATGTGTCACGCCCGCATTTGGTCAAGATGCTGGAGGAAGG
CGCCATCCCCTTTACAAAAACGGGCCGACATCGGCGAGTCCGTTTTTCGGATTTGATGGCGTTCAAGCAACGGCGTGACG
AGCAAAGCCAAGAGGCAATGGAAGCGCTGGTTCAACAGGCCCAAGAGTTGGGAATGGGGTATGACGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3E1I3F8

Antitoxin

Source ID Structure
AlphaFold DB A0A0W0MVK8

References