Detailed information of TA system TA066436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 649694..650216
• Replicon: chromosome
• Accession: NZ_CP017296
• Organism: Pseudomonas fluorescens strain Pt14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BH711_RS02960
• Protein ID: WP_038449426.1
• Coordinates: 649694..649987 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0W0N4Z1
• Locus tag: BH711_RS02965
• Protein ID: WP_014717442.1
• Coordinates: 649977..650216 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH711_RS02930	645087..645647	-	561	WP_003189463.1	glycine cleavage system protein R	-
BH711_RS02935	645910..646788	+	879	WP_038449421.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BH711_RS02940	646806..647921	+	1116	WP_014717369.1	outer membrane protein assembly factor BamC	-
BH711_RS02945	647926..648684	+	759	WP_014717370.1	MBL fold metallo-hydrolase	-
BH711_RS02950	648712..649425	+	714	WP_005786030.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BH711_RS02960	649694..649987	-	294	WP_038449426.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BH711_RS02965	649977..650216	-	240	WP_014717442.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BH711_RS02970	650623..650877	+	255	WP_032903173.1	hypothetical protein	-
BH711_RS02975	650951..652177	-	1227	WP_014717445.1	MFS transporter	-
BH711_RS02980	652422..652709	-	288	WP_014717446.1	helix-turn-helix domain-containing protein	-
BH711_RS02985	652712..653026	-	315	WP_038449428.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BH711_RS02990	653133..654074	-	942	WP_014717448.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11603.38 Da
• Isoelectric Point: 10.0977

>T66436 WP_038449426.1 NZ_CP017296:c649987-649694 [Pseudomonas fluorescens]
MTYSLDFDARALKEWQKLGDTLRQQLKKKLVEILNNPRIEANRLHRLPDCYKIKLRSGGYRLVYQVIDQEITVFVVAVDK
HERDQAYRKASERLDQR

Nucleotide

• Length: 294 bp

>T66436 NZ_CP017296:c649987-649694 [Pseudomonas fluorescens]
ATGACCTATAGCCTTGATTTTGATGCGCGTGCATTGAAAGAATGGCAAAAGCTCGGTGACACCCTCCGTCAGCAACTCAA
AAAGAAGCTGGTCGAAATCCTGAACAACCCACGTATTGAGGCTAATCGCCTACATCGCCTGCCTGACTGCTACAAGATCA
AACTGCGGAGTGGCGGGTACAGGCTGGTGTATCAAGTGATCGATCAGGAAATTACGGTGTTCGTGGTCGCCGTTGATAAA
CATGAGCGTGATCAGGCTTATCGCAAAGCGTCTGAGCGCCTTGATCAGCGTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8997.40 Da
• Isoelectric Point: 4.6017

>AT66436 WP_014717442.1 NZ_CP017296:c650216-649977 [Pseudomonas fluorescens]
MASINIRIDDDLKARAYLELEKLGVTPSELIRQALQYVAERGQLPFKPVLMTEEDEALMATVRERLASPQRVRVSLDDL

Nucleotide

• Length: 240 bp

>AT66436 NZ_CP017296:c650216-649977 [Pseudomonas fluorescens]
TTGGCATCTATCAACATTCGTATCGATGACGACCTGAAAGCTCGCGCCTACCTGGAGCTGGAGAAACTTGGTGTCACCCC
TTCCGAACTGATACGCCAGGCGTTGCAATATGTTGCCGAGCGCGGCCAGTTGCCATTTAAGCCTGTGCTGATGACTGAAG
AGGATGAAGCCTTGATGGCGACCGTACGTGAGCGTCTTGCATCGCCTCAACGCGTAAGGGTTTCTTTGGATGACCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0W0N4Z1