Detailed information of TA system TA066435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 222693..223140
• Replicon: chromosome
• Accession: NZ_CP017296
• Organism: Pseudomonas fluorescens strain Pt14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BH711_RS26300
• Protein ID: WP_081088256.1
• Coordinates: 222871..223140 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0W0ND88
• Locus tag: BH711_RS01030
• Protein ID: WP_014717050.1
• Coordinates: 222693..222881 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH711_RS01005	218232..219563	+	1332	WP_014717046.1	aromatic acid/H+ symport family MFS transporter	-
BH711_RS01010	219549..220352	-	804	WP_020301219.1	tetratricopeptide repeat protein	-
BH711_RS01015	220540..221559	+	1020	WP_014717048.1	Glu/Leu/Phe/Val dehydrogenase	-
BH711_RS01020	221615..221875	-	261	WP_003188807.1	YebG family protein	-
BH711_RS01025	222296..222541	+	246	WP_014717049.1	hypothetical protein	-
BH711_RS01030	222693..222881	+	189	WP_014717050.1	hypothetical protein	Antitoxin
BH711_RS26300	222871..223140	+	270	WP_081088256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BH711_RS01035	223310..223798	+	489	WP_014717052.1	phosphate-starvation-inducible PsiE family protein	-
BH711_RS01040	223795..224112	-	318	WP_014717053.1	DUF3509 domain-containing protein	-
BH711_RS01045	224378..225667	-	1290	WP_014717054.1	HAAAP family serine/threonine permease	-
BH711_RS01050	225729..227105	-	1377	WP_014717055.1	L-serine ammonia-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10355.16 Da
• Isoelectric Point: 10.9886

>T66435 WP_081088256.1 NZ_CP017296:222871-223140 [Pseudomonas fluorescens]
MLIEWSPAARVELRQIIEYLSHRNPVAALELKRSIEASVLALSRRPHLYRPGRIGGTREMVVHPNYLVVYKVTDNIRILS
VLHARQRYP

Nucleotide

• Length: 270 bp

>T66435 NZ_CP017296:222871-223140 [Pseudomonas fluorescens]
ATGCTGATTGAGTGGTCGCCCGCAGCGCGGGTAGAACTCCGGCAAATTATTGAGTACCTCAGTCATCGTAATCCTGTAGC
AGCGCTTGAGCTTAAGCGATCAATCGAAGCTTCAGTGCTTGCCCTCTCGCGCAGACCCCATCTTTACCGCCCCGGCAGAA
TCGGCGGAACACGAGAGATGGTGGTACATCCGAATTATCTGGTTGTATACAAAGTGACAGACAATATTCGGATTCTCTCG
GTTTTACACGCACGCCAGCGATATCCTTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6884.55 Da
• Isoelectric Point: 4.0061

>AT66435 WP_014717050.1 NZ_CP017296:222693-222881 [Pseudomonas fluorescens]
MDTSFSSGIQADIEEQADSYDRWFRARVQAALDDPSPGIPHDEVMAEMQALIESKLKKGDAD

Nucleotide

• Length: 189 bp

>AT66435 NZ_CP017296:222693-222881 [Pseudomonas fluorescens]
ATGGACACTTCCTTTTCCTCAGGTATCCAAGCAGATATCGAAGAGCAGGCTGACAGCTACGACCGCTGGTTTCGCGCGAG
AGTGCAGGCCGCTCTTGATGACCCAAGCCCTGGCATTCCTCACGACGAGGTGATGGCCGAGATGCAAGCGCTGATCGAAT
CTAAACTCAAGAAAGGCGATGCTGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0W0ND88