Detailed information of TA system TA066432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 18091..18784
• Replicon: plasmid unnamed1
• Accession: NZ_CP017294
• Organism: Pseudomonas aeruginosa strain PA83

Toxin (Protein)

• Gene name: tad
• Uniprot ID: N2IHR9
• Locus tag: PSA83_RS32340
• Protein ID: WP_003151133.1
• Coordinates: 18091..18468 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: PSA83_RS32345
• Protein ID: WP_001172026.1
• Coordinates: 18449..18784 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSA83_RS32325	14022..14258	+	237	WP_151904090.1	helix-turn-helix domain-containing protein	-
PSA83_RS32330	14281..17310	-	3030	WP_012077404.1	Tn3 family transposase	-
PSA83_RS32335	17307..17900	-	594	WP_012435588.1	recombinase family protein	-
PSA83_RS32340	18091..18468	+	378	WP_003151133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSA83_RS32345	18449..18784	+	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
PSA83_RS32350	18799..19134	+	336	WP_000741275.1	hypothetical protein	-
PSA83_RS32355	19158..19484	+	327	WP_000091614.1	hypothetical protein	-
PSA83_RS32360	19481..19861	+	381	WP_001054412.1	hypothetical protein	-
PSA83_RS32365	19979..20296	+	318	WP_079455029.1	DUF3391 domain-containing protein	-
PSA83_RS32370	20335..21249	+	915	WP_001736514.1	HD domain-containing protein	-
PSA83_RS32375	21562..22263	+	702	WP_024161570.1	CerR family C-terminal domain-containing protein	-
PSA83_RS32380	22301..22909	+	609	WP_003296513.1	hypothetical protein	-
PSA83_RS32385	22958..23665	+	708	WP_003296512.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..398087	398087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.78 Da
• Isoelectric Point: 9.4693

>T66432 WP_003151133.1 NZ_CP017294:18091-18468 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T66432 NZ_CP017294:18091-18468 [Pseudomonas aeruginosa]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT66432 WP_001172026.1 NZ_CP017294:18449-18784 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT66432 NZ_CP017294:18449-18784 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A024ELN5

Antitoxin

• AlphaFold DB: A0A024EKI7