Detailed information of TA system TA066427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2503200..2503716
• Replicon: chromosome
• Accession: NZ_CP017293
• Organism: Pseudomonas aeruginosa strain PA83

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PSA83_RS12080
• Protein ID: WP_071557699.1
• Coordinates: 2503435..2503716 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A482UCX2
• Locus tag: PSA83_RS12075
• Protein ID: WP_033942931.1
• Coordinates: 2503200..2503445 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSA83_RS12065	2498926..2502093	+	3168	WP_033942936.1	DEAD/DEAH box helicase	-
PSA83_RS34865	2502217..2502675	+	459	Protein_2332	hypothetical protein	-
PSA83_RS34780	2502593..2503030	-	438	WP_033942934.1	hypothetical protein	-
PSA83_RS12075	2503200..2503445	+	246	WP_033942931.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PSA83_RS12080	2503435..2503716	+	282	WP_071557699.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSA83_RS12085	2503970..2504467	-	498	WP_033942928.1	DNA repair protein RadC	-
PSA83_RS12090	2504478..2505389	-	912	WP_033942927.1	hypothetical protein	-
PSA83_RS12095	2505482..2506486	-	1005	WP_033942926.1	YqaJ viral recombinase family protein	-
PSA83_RS12100	2506561..2507529	-	969	WP_033942925.1	DUF932 domain-containing protein	-
PSA83_RS12110	2508335..2508553	+	219	WP_071557701.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10721.55 Da
• Isoelectric Point: 10.6485

>T66427 WP_071557699.1 NZ_CP017293:2503435-2503716 [Pseudomonas aeruginosa]
MTYKLEFLPSALKEWEKLGHTVREQAKKKLRERLEAPKVQADALRDLPGHYKIKLRTAGYRLVYNVEDDRVVVIVVAVGK
RERSAAYKAAGKR

Nucleotide

• Length: 282 bp

>T66427 NZ_CP017293:2503435-2503716 [Pseudomonas aeruginosa]
ATGACCTATAAGCTCGAGTTCCTGCCCTCAGCGCTGAAGGAATGGGAGAAGCTGGGTCATACGGTGCGTGAGCAGGCAAA
GAAAAAACTGCGTGAGCGGCTGGAAGCACCAAAGGTACAGGCTGATGCCCTCAGGGATCTGCCTGGCCACTACAAGATCA
AGCTCCGAACAGCAGGCTATCGATTGGTCTATAATGTCGAGGATGATCGGGTGGTTGTGATTGTGGTCGCAGTGGGCAAG
CGTGAACGAAGTGCAGCTTACAAGGCAGCAGGTAAGCGTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8653.98 Da
• Isoelectric Point: 4.1469

>AT66427 WP_033942931.1 NZ_CP017293:2503200-2503445 [Pseudomonas aeruginosa]
MQSVLADVAVSVSELKKNPTGVMAGAGGMPVAVLNHNRVMGYMVPAELYEQMLDRLDDLDLIDVVNARSGEKGVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT66427 NZ_CP017293:2503200-2503445 [Pseudomonas aeruginosa]
ATGCAGAGTGTTTTGGCTGACGTTGCCGTGAGCGTTTCTGAGCTGAAGAAAAACCCCACCGGTGTCATGGCTGGTGCGGG
TGGCATGCCGGTCGCAGTTCTCAATCATAACCGCGTGATGGGGTACATGGTGCCAGCCGAGCTGTACGAGCAGATGTTGG
ACCGTTTGGACGATCTGGATCTGATCGATGTCGTTAACGCGCGCTCCGGTGAGAAGGGTGTGCCGGTGAACCTGGATGAC
CTATAA

Structures

Antitoxin

• AlphaFold DB: A0A482UCX2