Detailed information of TA system TA066421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2487546..2489141
• Replicon: chromosome
• Accession: NZ_CP017290
• Organism: Pseudomonas sp. LPH1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BHQ29_RS11335
• Protein ID: WP_079783795.1
• Coordinates: 2487546..2488871 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1G5YAZ4
• Locus tag: BHQ29_RS11340
• Protein ID: WP_017676963.1
• Coordinates: 2488872..2489141 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHQ29_RS11310	2483315..2484082	-	768	WP_079783792.1	sulfite exporter TauE/SafE family protein	-
BHQ29_RS11315	2484182..2485144	+	963	WP_039964599.1	LysR family transcriptional regulator	-
BHQ29_RS11320	2485229..2485801	-	573	WP_003460252.1	elongation factor P	-
BHQ29_RS11325	2485836..2486978	-	1143	WP_079783793.1	elongation factor P maturation arginine rhamnosyltransferase EarP	-
BHQ29_RS11330	2487087..2487293	-	207	WP_079783794.1	hypothetical protein	-
BHQ29_RS11335	2487546..2488871	+	1326	WP_079783795.1	type II toxin-antitoxin system HipA family toxin	Toxin
BHQ29_RS11340	2488872..2489141	+	270	WP_017676963.1	helix-turn-helix domain-containing protein	Antitoxin
BHQ29_RS11345	2489135..2489344	-	210	WP_079783796.1	hypothetical protein	-
BHQ29_RS11350	2489466..2490434	-	969	WP_074914922.1	LLM class oxidoreductase	-
BHQ29_RS11355	2490655..2492436	-	1782	WP_079783797.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
BHQ29_RS11360	2492759..2493229	-	471	WP_021441646.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-382)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 49113.49 Da
• Isoelectric Point: 5.8310

>T66421 WP_079783795.1 NZ_CP017290:2487546-2488871 [Pseudomonas sp. LPH1]
MHSLTLQLYSGHAWHDAMHFSFEQPEAGLAGACSFGYEQAYLLETLEHIGSRLEHAVSVTVPLDWESYRASEAPAFLHDI
LPAGAARRFLLQRLGDQKPESLGLDLFLLSRCAPAPVGNLRIKEAAAALQESPLLGFSREDVINRDSRFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDREGALHPDAALPDESATRHWFVKFARNQARQTDQDILRSEFCYYRAIQRLGLDTVPAEGLAL
EEGRKPSLWMHRFDRRLSAAGVERSAMESLYSLAGVTVPGSYLLHTDALHHLVRLWQKTGQGAQVGDMIFEYLQRDLLNQ
ILGNSDNHGRNTSILRNSTGLKLAPIYDLAPMVMDDEGVTRTTKWPQSIEIAGEVNWRQACAALAQWTDADVLYERLRGA
AQDFLALPDLLSDQGLPNSTMNHPRIALRTLHQRLNRWELV

Nucleotide

• Length: 1326 bp

>T66421 NZ_CP017290:2487546-2488871 [Pseudomonas sp. LPH1]
ATGCACTCTCTCACCCTGCAACTGTACAGTGGCCATGCCTGGCACGATGCCATGCACTTCAGCTTCGAGCAGCCCGAGGC
GGGTCTGGCGGGGGCGTGTTCTTTTGGCTATGAGCAGGCATATCTGCTTGAAACGCTGGAGCACATTGGCAGCCGCCTTG
AGCACGCAGTTAGCGTCACAGTGCCGTTGGACTGGGAATCCTATCGAGCAAGCGAAGCGCCTGCCTTCCTGCACGACATA
CTGCCCGCTGGAGCAGCAAGGCGCTTCCTTCTCCAGCGCCTGGGTGACCAAAAGCCCGAGAGTCTTGGACTGGATCTATT
TTTGCTAAGTCGGTGCGCACCAGCGCCGGTGGGAAACCTGCGTATCAAGGAGGCAGCAGCAGCGCTTCAGGAAAGTCCGC
TGTTAGGCTTCTCTCGCGAGGATGTAATCAACCGCGACTCGCGCTTTCTGGAGTACGCCTACGAGCAAGGTGCGGCTATC
GGCGGCGCCACAGGCGCAGGTGGAGAAGCACCCAAATTATTGCTGACTGAAGACCGAGAAGGCGCCCTGCACCCAGATGC
GGCTTTGCCGGATGAGTCAGCAACCCGCCACTGGTTTGTAAAGTTCGCGCGAAACCAGGCCAGGCAGACCGACCAAGACA
TCCTGCGTAGCGAGTTCTGCTACTACAGAGCCATTCAGCGGCTTGGCCTGGATACCGTACCCGCAGAAGGCCTGGCGCTA
GAGGAAGGCCGCAAGCCTAGCCTGTGGATGCATCGCTTTGATCGCCGTCTCAGCGCCGCAGGCGTAGAGCGCAGCGCAAT
GGAGTCCCTGTACTCACTCGCAGGAGTAACCGTGCCGGGCAGCTACCTGCTGCACACCGACGCCCTGCATCACTTGGTCC
GGCTGTGGCAGAAGACGGGGCAAGGCGCGCAAGTTGGCGACATGATCTTTGAGTATCTACAGCGAGACTTACTCAACCAG
ATCCTTGGCAACTCGGACAACCACGGCCGCAATACCTCGATTTTGCGCAACAGCACGGGGCTGAAACTCGCCCCTATCTA
CGACCTTGCGCCCATGGTGATGGATGATGAAGGCGTCACTCGCACCACAAAATGGCCTCAATCCATAGAGATTGCCGGCG
AGGTCAACTGGCGCCAAGCCTGCGCCGCACTAGCGCAATGGACCGACGCAGATGTTCTTTATGAAAGACTCAGAGGCGCA
GCACAAGACTTCCTGGCCCTTCCCGACCTACTGAGCGATCAAGGGTTGCCAAACTCCACGATGAATCATCCGCGTATTGC
GCTGCGCACCCTTCATCAAAGACTCAACCGATGGGAGCTTGTCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10104.75 Da
• Isoelectric Point: 10.7429

>AT66421 WP_017676963.1 NZ_CP017290:2488872-2489141 [Pseudomonas sp. LPH1]
MDIIERNRILTEIQTQLASGELTIGQAVRKLRKEITGLQQARFAQMCKLSLRALRQLEHDESNPTVQTLNSVFNPFGMQV
GIVPKSRIQ

Nucleotide

• Length: 270 bp

>AT66421 NZ_CP017290:2488872-2489141 [Pseudomonas sp. LPH1]
ATGGATATCATCGAGCGCAACCGTATTCTGACTGAAATACAGACCCAACTGGCATCGGGCGAGTTGACCATCGGCCAGGC
CGTGCGCAAATTACGCAAGGAAATCACCGGATTGCAGCAGGCCCGTTTCGCACAGATGTGCAAACTGTCCCTGCGTGCCC
TTCGTCAATTGGAACATGACGAGTCGAATCCAACCGTGCAGACCCTCAATAGCGTGTTCAATCCATTTGGGATGCAAGTT
GGCATTGTGCCGAAGAGTCGAATTCAATAG

Structures

Antitoxin

• AlphaFold DB: A0A1G5YAZ4