Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1263096..1263715 Replicon chromosome
Accession NZ_CP017289
Organism Klebsiella variicola strain GJ3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BBD65_RS08310 Protein ID WP_002892050.1
Coordinates 1263096..1263314 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag BBD65_RS08315 Protein ID WP_008805436.1
Coordinates 1263341..1263715 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD65_RS08280 1259139..1259402 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
BBD65_RS08285 1259402..1259542 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BBD65_RS08290 1259539..1260237 - 699 WP_012968815.1 GNAT family N-acetyltransferase -
BBD65_RS08295 1260338..1261795 + 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
BBD65_RS08300 1261764..1262234 - 471 WP_008805434.1 YlaC family protein -
BBD65_RS30750 1262271..1262399 + 129 Protein_1139 hypothetical protein -
BBD65_RS08305 1262371..1262937 - 567 WP_032438710.1 maltose O-acetyltransferase -
BBD65_RS08310 1263096..1263314 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BBD65_RS08315 1263341..1263715 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
BBD65_RS08325 1264198..1267344 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
BBD65_RS08330 1267367..1268560 - 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-69)

Antitoxin

(1-116)


Sequences


Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T66403 WP_002892050.1 NZ_CP017289:c1263314-1263096 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T66403 NZ_CP017289:c1263314-1263096 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT66403 WP_008805436.1 NZ_CP017289:c1263715-1263341 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT66403 NZ_CP017289:c1263715-1263341 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2


Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References