Detailed information of TA system TA066391

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4345258..4346041
• Replicon: chromosome
• Accession: NZ_CP017284
• Organism: Klebsiella variicola strain GJ1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BBD63_RS22925
• Protein ID: WP_167353669.1
• Coordinates: 4345673..4346041 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BBD63_RS22920
• Protein ID: WP_040018969.1
• Coordinates: 4345258..4345617 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD63_RS22875	4340475..4341332	+	858	WP_032705139.1	hypothetical protein	-
BBD63_RS22880	4341610..4342074	+	465	WP_004114621.1	hypothetical protein	-
BBD63_RS22885	4342129..4342488	+	360	WP_004114622.1	hypothetical protein	-
BBD63_RS22890	4342574..4342786	+	213	WP_004114623.1	hypothetical protein	-
BBD63_RS22895	4342897..4343718	+	822	WP_040018972.1	DUF945 domain-containing protein	-
BBD63_RS22900	4343798..4344277	+	480	WP_040018971.1	antirestriction protein	-
BBD63_RS22910	4344518..4345000	+	483	WP_004114630.1	DNA repair protein RadC	-
BBD63_RS22915	4345014..4345235	+	222	WP_004114632.1	DUF987 domain-containing protein	-
BBD63_RS22920	4345258..4345617	+	360	WP_040018969.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BBD63_RS22925	4345673..4346041	+	369	WP_167353669.1	TA system toxin CbtA family protein	Toxin
BBD63_RS22930	4346121..4346441	-	321	WP_004114640.1	hypothetical protein	-
BBD63_RS22935	4346580..4347569	-	990	WP_004114642.1	glycosyltransferase	-
BBD63_RS22945	4348192..4349109	-	918	WP_004114644.1	hypothetical protein	-
BBD63_RS22950	4349196..4350098	+	903	WP_077139515.1	bacteriophage abortive infection AbiH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4321002..4363353	42351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (7-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.52 Da
• Isoelectric Point: 9.4643

>T66391 WP_167353669.1 NZ_CP017284:4345673-4346041 [Klebsiella variicola]
IQSLPPQRAASSRLSSVEIWQKLLEYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGFSV
KEQSPFIGSLDMLRARKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 369 bp

>T66391 NZ_CP017284:4345673-4346041 [Klebsiella variicola]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACGACACGCCGTTTGGCAACGACAGTGTGATCCAGAAACACATTGATGCGG
GAATCTCACTGTGTGACGCTGTAAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGCCCGGAAGGCTACCGGGCTGATGACCCGCAAGGGATA
TAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13349.56 Da
• Isoelectric Point: 8.1516

>AT66391 WP_040018969.1 NZ_CP017284:4345258-4345617 [Klebsiella variicola]
MKKATRIINHNIAEPWWGLRRNITPCLGARLVQEGNRLHYLADRASIAGTFNDTDLRHLDQAFPVLMKQMELMLASSELT
PHIQRCITLHAKGLICEADTLGSCGYLYIVIYPTSAITA

Nucleotide

• Length: 360 bp

>AT66391 NZ_CP017284:4345258-4345617 [Klebsiella variicola]
ATGAAGAAAGCAACCCGTATAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACA
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAGCAGCGAACTCACG
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCTGATACGCTCGGCTCCTGTGGCTATTT
GTATATCGTAATTTATCCGACATCTGCAATAACCGCATAA