Detailed information of TA system TA066378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 523742..524258
• Replicon: chromosome
• Accession: NZ_CP017284
• Organism: Klebsiella variicola strain GJ1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BBD63_RS04215
• Protein ID: WP_022065401.1
• Coordinates: 523974..524258 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: BBD63_RS04210
• Protein ID: WP_002886901.1
• Coordinates: 523742..523984 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD63_RS04195	519711..520451	+	741	WP_022065398.1	KDGP aldolase family protein	-
BBD63_RS04200	520577..521731	+	1155	WP_012969087.1	lactonase family protein	-
BBD63_RS04205	521754..523664	+	1911	WP_032731805.1	BglG family transcription antiterminator	-
BBD63_RS04210	523742..523984	+	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BBD63_RS04215	523974..524258	+	285	WP_022065401.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBD63_RS04220	524262..524726	-	465	WP_022065402.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
BBD63_RS04225	524976..527114	-	2139	WP_077139001.1	anaerobic ribonucleoside-triphosphate reductase	-
BBD63_RS04230	527500..529155	-	1656	WP_077139002.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11128.97 Da
• Isoelectric Point: 10.2849

>T66378 WP_022065401.1 NZ_CP017284:523974-524258 [Klebsiella variicola]
MTYELEFDPRAWREWQMPGETVKKQFKNKLQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T66378 NZ_CP017284:523974-524258 [Klebsiella variicola]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGATGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT66378 WP_002886901.1 NZ_CP017284:523742-523984 [Klebsiella variicola]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT66378 NZ_CP017284:523742-523984 [Klebsiella variicola]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0