Detailed information of TA system TA066375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 17182..17707
• Replicon: plasmid pKPGJ-1b
• Accession: NZ_CP017281
• Organism: Klebsiella variicola strain GJ1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: BBD63_RS00105
• Protein ID: WP_012539978.1
• Coordinates: 17402..17707 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: BBD63_RS00100
• Protein ID: WP_015632547.1
• Coordinates: 17182..17400 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD63_RS00080	12709..12963	-	255	WP_032700773.1	hypothetical protein	-
BBD63_RS00085	13243..15492	-	2250	WP_077138867.1	hypothetical protein	-
BBD63_RS00090	15494..16588	-	1095	WP_077138868.1	transcriptional regulator	-
BBD63_RS00100	17182..17400	+	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BBD63_RS00105	17402..17707	+	306	WP_012539978.1	type II toxin-antitoxin system toxin CcdB	Toxin
BBD63_RS00110	17894..18898	+	1005	WP_077138869.1	hypothetical protein	-
BBD63_RS00115	19096..19875	+	780	WP_029497485.1	site-specific integrase	-
BBD63_RS00120	19933..20190	-	258	WP_032433931.1	hypothetical protein	-
BBD63_RS30055	20318..20431	-	114	WP_032072094.1	hypothetical protein	-
BBD63_RS00125	21063..21818	+	756	WP_012539983.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92219	92219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.27 Da
• Isoelectric Point: 6.4661

>T66375 WP_012539978.1 NZ_CP017281:17402-17707 [Klebsiella variicola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T66375 NZ_CP017281:17402-17707 [Klebsiella variicola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT66375 WP_015632547.1 NZ_CP017281:17182-17400 [Klebsiella variicola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT66375 NZ_CP017281:17182-17400 [Klebsiella variicola]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86