Detailed information of TA system TA066374

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4799454..4800237
• Replicon: chromosome
• Accession: NZ_CP017279
• Organism: Enterobacter ludwigii strain EN-119

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BH714_RS22605
• Protein ID: WP_167353669.1
• Coordinates: 4799454..4799822 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BH714_RS22610
• Protein ID: WP_040018969.1
• Coordinates: 4799878..4800237 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH714_RS22585	4795397..4796341	-	945	WP_199660085.1	bacteriophage abortive infection AbiH family protein	-
BH714_RS22590	4796386..4797303	+	918	WP_040018967.1	hypothetical protein	-
BH714_RS22595	4797926..4798915	+	990	WP_004114642.1	glycosyltransferase	-
BH714_RS22600	4799054..4799374	+	321	WP_004114640.1	hypothetical protein	-
BH714_RS22605	4799454..4799822	-	369	WP_167353669.1	TA system toxin CbtA family protein	Toxin
BH714_RS22610	4799878..4800237	-	360	WP_040018969.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BH714_RS22615	4800260..4800481	-	222	WP_004114632.1	DUF987 domain-containing protein	-
BH714_RS22620	4800495..4800977	-	483	WP_004114630.1	DNA repair protein RadC	-
BH714_RS22625	4800974..4801207	-	234	WP_040018970.1	hypothetical protein	-
BH714_RS22630	4801218..4801697	-	480	WP_040018971.1	antirestriction protein	-
BH714_RS22635	4801777..4802598	-	822	WP_040018972.1	DUF945 domain-containing protein	-
BH714_RS22640	4802709..4802921	-	213	WP_040018973.1	hypothetical protein	-
BH714_RS22645	4803007..4803366	-	360	WP_004114622.1	hypothetical protein	-
BH714_RS22650	4803421..4803885	-	465	WP_004114621.1	hypothetical protein	-
BH714_RS22655	4804163..4805020	-	858	WP_032705139.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4782142..4828323	46181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (7-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.52 Da
• Isoelectric Point: 9.4643

>T66374 WP_167353669.1 NZ_CP017279:c4799822-4799454 [Enterobacter ludwigii]
IQSLPPQRAASSRLSSVEIWQKLLEYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGFSV
KEQSPFIGSLDMLRARKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 369 bp

>T66374 NZ_CP017279:c4799822-4799454 [Enterobacter ludwigii]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACGACACGCCGTTTGGCAACGACAGTGTGATCCAGAAACACATTGATGCGG
GAATCTCACTGTGTGACGCTGTAAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGCCCGGAAGGCTACCGGGCTGATGACCCGCAAGGGATA
TAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13349.56 Da
• Isoelectric Point: 8.1516

>AT66374 WP_040018969.1 NZ_CP017279:c4800237-4799878 [Enterobacter ludwigii]
MKKATRIINHNIAEPWWGLRRNITPCLGARLVQEGNRLHYLADRASIAGTFNDTDLRHLDQAFPVLMKQMELMLASSELT
PHIQRCITLHAKGLICEADTLGSCGYLYIVIYPTSAITA

Nucleotide

• Length: 360 bp

>AT66374 NZ_CP017279:c4800237-4799878 [Enterobacter ludwigii]
ATGAAGAAAGCAACCCGTATAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACA
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAGCAGCGAACTCACG
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCTGATACGCTCGGCTCCTGTGGCTATTT
GTATATCGTAATTTATCCGACATCTGCAATAACCGCATAA