Detailed information of TA system TA066371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3590743..3591259
• Replicon: chromosome
• Accession: NZ_CP017279
• Organism: Enterobacter ludwigii strain EN-119

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BH714_RS16925
• Protein ID: WP_040018490.1
• Coordinates: 3590975..3591259 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A839BTQ9
• Locus tag: BH714_RS16920
• Protein ID: WP_020885404.1
• Coordinates: 3590743..3590985 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH714_RS16905	3586740..3587480	+	741	WP_040018487.1	KDGP aldolase family protein	-
BH714_RS16910	3587599..3588735	+	1137	WP_040018489.1	lactonase family protein	-
BH714_RS16915	3588755..3590665	+	1911	WP_014168384.1	BglG family transcription antiterminator	-
BH714_RS16920	3590743..3590985	+	243	WP_020885404.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BH714_RS16925	3590975..3591259	+	285	WP_040018490.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BH714_RS16930	3591263..3591727	-	465	WP_040018491.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
BH714_RS16935	3591866..3594004	-	2139	WP_020885401.1	anaerobic ribonucleoside-triphosphate reductase	-
BH714_RS16940	3594372..3594944	-	573	WP_032679517.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10886.64 Da
• Isoelectric Point: 10.0775

>T66371 WP_040018490.1 NZ_CP017279:3590975-3591259 [Enterobacter ludwigii]
MTYELVFDPRAFKEWTRLGDTVKNQFKKKLANVLVNPRVESARLYGLPDCDKIKLRSQGYSLVYQVHDNVVTVCVITIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T66371 NZ_CP017279:3590975-3591259 [Enterobacter ludwigii]
ATGACTTATGAACTCGTATTTGATCCGAGGGCGTTCAAGGAGTGGACCAGGCTGGGTGATACCGTAAAAAATCAGTTTAA
GAAGAAGCTTGCGAACGTGTTGGTGAACCCCAGGGTTGAATCGGCTCGCCTGTATGGCCTGCCTGATTGCGACAAGATAA
AACTCAGATCCCAGGGTTATAGTTTGGTCTATCAGGTTCATGACAACGTTGTGACGGTATGTGTTATTACTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGACGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9085.49 Da
• Isoelectric Point: 5.1088

>AT66371 WP_020885404.1 NZ_CP017279:3590743-3590985 [Enterobacter ludwigii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDELIQTVRERLANPQKGIKVRLDDL

Nucleotide

• Length: 243 bp

>AT66371 NZ_CP017279:3590743-3590985 [Enterobacter ludwigii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACGCC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCGTATGCCGGTGAAAACGGTCACTGTTAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAATTAAGGTTAGGCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A839BTQ9