Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4357018..4357645 Replicon chromosome
Accession NZ_CP017269
Organism Geosporobacter ferrireducens strain IRF9

Toxin (Protein)


Gene name mazF Uniprot ID A0A1M6D7W3
Locus tag Gferi_RS20490 Protein ID WP_069979814.1
Coordinates 4357295..4357645 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1D8GLB9
Locus tag Gferi_RS20485 Protein ID WP_069979812.1
Coordinates 4357018..4357293 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Gferi_RS20460 4352292..4352711 + 420 WP_069979805.1 holo-ACP synthase -
Gferi_RS20465 4352695..4354248 + 1554 WP_069979806.1 NAD(P)H-hydrate dehydratase -
Gferi_RS20470 4354262..4354708 + 447 WP_069979808.1 CBS domain-containing protein -
Gferi_RS20475 4354758..4355399 + 642 WP_069979810.1 outer membrane lipoprotein carrier protein LolA -
Gferi_RS20480 4355538..4356713 + 1176 WP_069979811.1 alanine racemase -
Gferi_RS20485 4357018..4357293 + 276 WP_069979812.1 ribbon-helix-helix protein, CopG family Antitoxin
Gferi_RS20490 4357295..4357645 + 351 WP_069979814.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
Gferi_RS20495 4357831..4358325 + 495 WP_069979819.1 hypothetical protein -
Gferi_RS20500 4358350..4359846 + 1497 WP_069979821.1 lipopolysaccharide biosynthesis protein -
Gferi_RS20505 4359833..4360321 + 489 WP_069979823.1 DUF4330 domain-containing protein -
Gferi_RS20510 4360335..4360871 + 537 WP_069979825.1 hypothetical protein -
Gferi_RS20515 4360873..4362225 + 1353 WP_069979827.1 O-antigen ligase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12817.83 Da        Isoelectric Point: 6.2293

>T66361 WP_069979814.1 NZ_CP017269:4357295-4357645 [Geosporobacter ferrireducens]
VIVKRGDIFYADLSPVIGSEQGGVRPVLIVQNDVGNKYSPTVIVAAITSQINKAKLPTHLEISATEYGLSKDSVVLLEQI
RTIDKKRLREKLGHLDEEMMHKVDEALSISFGLVDF

Download         Length: 351 bp

>T66361 NZ_CP017269:4357295-4357645 [Geosporobacter ferrireducens]
GTGATTGTAAAAAGAGGCGATATTTTTTATGCGGATCTCAGTCCTGTGATTGGTTCGGAACAAGGCGGTGTCAGACCTGT
TCTCATTGTTCAAAATGATGTTGGAAATAAATACAGCCCTACAGTGATTGTAGCGGCGATTACTTCTCAGATCAATAAGG
CAAAGCTACCGACGCATTTGGAGATAAGTGCCACAGAATATGGATTGTCAAAGGATTCCGTTGTATTGCTAGAGCAAATT
CGGACGATTGACAAGAAAAGACTTCGGGAAAAGCTCGGACATTTGGATGAAGAGATGATGCATAAAGTGGATGAAGCGTT
ATCCATCAGCTTTGGATTAGTAGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10594.28 Da        Isoelectric Point: 6.3697

>AT66361 WP_069979812.1 NZ_CP017269:4357018-4357293 [Geosporobacter ferrireducens]
VAESKRIIISLPDSLLREVDDIVEVEKRNRSECIREAMRLYIRERKKIQIRETMKKGYREMGAINLALAETGIYSDFQTL
EGYVAKLAECE

Download         Length: 276 bp

>AT66361 NZ_CP017269:4357018-4357293 [Geosporobacter ferrireducens]
GTGGCTGAGTCAAAGCGTATTATAATAAGCTTGCCAGACAGTTTATTGAGAGAAGTAGATGATATTGTCGAAGTGGAGAA
ACGAAACCGAAGCGAATGTATCCGTGAAGCGATGCGGTTGTATATTCGGGAAAGAAAGAAAATACAAATTCGTGAAACCA
TGAAGAAGGGCTACAGGGAGATGGGAGCGATTAATTTAGCCTTAGCTGAAACAGGTATATATTCGGATTTTCAAACACTC
GAAGGCTATGTAGCAAAGCTAGCGGAGTGTGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M6D7W3

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8GLB9

References