Detailed information of TA system TA066353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 44012..44571
• Replicon: type plasmid pO157
• Accession: NZ_CP017252
• Organism: Escherichia coli strain NADC 5570/86-24/6564 isolate wild

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: BHW77_RS28115
• Protein ID: WP_000421248.1
• Coordinates: 44012..44287 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: BHW77_RS28120
• Protein ID: WP_001178089.1
• Coordinates: 44287..44571 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHW77_RS28080	39365..39754	-	390	WP_001172748.1	cytochrome b562 family protein	-
BHW77_RS28085	39798..42008	-	2211	WP_000592771.1	catalase/peroxidase KatP	-
BHW77_RS28090	42185..42370	+	186	WP_001302179.1	hypothetical protein	-
BHW77_RS28095	42370..42786	+	417	Protein_35	IS91 family transposase	-
BHW77_RS30830	42786..43352	+	567	Protein_36	IS3-like element IS600 family transposase	-
BHW77_RS28105	43396..43611	+	216	Protein_37	transposase	-
BHW77_RS28110	43711..43917	-	207	WP_001105064.1	hypothetical protein	-
BHW77_RS28115	44012..44287	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BHW77_RS28120	44287..44571	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
BHW77_RS28125	45484..46341	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
BHW77_RS28130	46334..46408	-	75	WP_001370046.1	RepA leader peptide Tap	-
BHW77_RS28135	46644..46898	-	255	WP_000083831.1	replication regulatory protein RepA	-
BHW77_RS28140	47138..47476	-	339	WP_000766796.1	hypothetical protein	-
BHW77_RS28145	47514..47723	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
BHW77_RS28150	47769..48230	-	462	WP_001233853.1	thermonuclease family protein	-
BHW77_RS28155	48475..48687	-	213	WP_001302189.1	ANR family transcriptional regulator	-
BHW77_RS28160	48820..49380	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB	1..92691	92691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T66353 WP_000421248.1 NZ_CP017252:c44287-44012 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T66353 NZ_CP017252:c44287-44012 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT66353 WP_001178089.1 NZ_CP017252:c44571-44287 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT66353 NZ_CP017252:c44571-44287 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8