Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4790719..4791554 Replicon chromosome
Accession NZ_CP017251
Organism Escherichia coli strain NADC 5570/86-24/6564 isolate wild

Toxin (Protein)


Gene name yeeV Uniprot ID B1LDL9
Locus tag BHW77_RS24620 Protein ID WP_001094444.1
Coordinates 4790719..4791096 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7LDN9
Locus tag BHW77_RS24625 Protein ID WP_001285607.1
Coordinates 4791186..4791554 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW77_RS24590 4786868..4787110 + 243 Protein_4664 N-acetylneuraminic acid mutarotase -
BHW77_RS24595 4787184..4788035 + 852 WP_000168589.1 HNH endonuclease -
BHW77_RS24600 4788233..4788420 - 188 Protein_4666 transposase -
BHW77_RS24605 4788495..4789322 - 828 WP_001301682.1 integrase arm-type DNA-binding domain-containing protein -
BHW77_RS32295 4789786..4789932 - 147 WP_001290178.1 hypothetical protein -
BHW77_RS24610 4790017..4790214 - 198 WP_001327226.1 DUF957 domain-containing protein -
BHW77_RS24615 4790234..4790722 - 489 WP_000761685.1 hypothetical protein -
BHW77_RS24620 4790719..4791096 - 378 WP_001094444.1 TA system toxin CbtA family protein Toxin
BHW77_RS24625 4791186..4791554 - 369 WP_001285607.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BHW77_RS24630 4791634..4791855 - 222 WP_000692350.1 DUF987 domain-containing protein -
BHW77_RS24635 4791942..4792418 - 477 WP_001186780.1 RadC family protein -
BHW77_RS24640 4792434..4792919 - 486 WP_000213716.1 antirestriction protein -
BHW77_RS24645 4793011..4793829 - 819 WP_001175175.1 DUF945 domain-containing protein -
BHW77_RS24650 4793919..4794128 - 210 WP_032150870.1 DUF905 family protein -
BHW77_RS24655 4794158..4794835 - 678 WP_001097301.1 hypothetical protein -
BHW77_RS24660 4794983..4795663 - 681 WP_001282919.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4783170..4828453 45283


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14087.02 Da        Isoelectric Point: 7.3523

>T66350 WP_001094444.1 NZ_CP017251:c4791096-4790719 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T66350 NZ_CP017251:c4791096-4790719 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13689.53 Da        Isoelectric Point: 6.4669

>AT66350 WP_001285607.1 NZ_CP017251:c4791554-4791186 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT66350 NZ_CP017251:c4791554-4791186 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B1LDL9

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0JLU8

References