Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 310093..310724 Replicon chromosome
Accession NZ_CP017251
Organism Escherichia coli strain NADC 5570/86-24/6564 isolate wild

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag BHW77_RS01510 Protein ID WP_001259386.1
Coordinates 310093..310368 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BHW77_RS01515 Protein ID WP_000593555.1
Coordinates 310365..310724 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW77_RS01495 305097..306164 + 1068 WP_000361458.1 HlyD family secretion protein -
BHW77_RS01500 306161..308896 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
BHW77_RS01505 308896..310020 + 1125 WP_001301659.1 ABC transporter permease -
BHW77_RS01510 310093..310368 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
BHW77_RS01515 310365..310724 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BHW77_RS01520 310774..311634 - 861 WP_001131788.1 class II aldolase -
BHW77_RS01525 311666..311935 - 270 WP_000933060.1 HPr family phosphocarrier protein -
BHW77_RS01530 311925..313433 - 1509 WP_001301773.1 carbohydrate kinase -
BHW77_RS01535 313426..314784 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
BHW77_RS01540 314861..315142 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
BHW77_RS01545 315139..315612 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 301237..310724 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T66323 WP_001259386.1 NZ_CP017251:310093-310368 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T66323 NZ_CP017251:310093-310368 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT66323 WP_000593555.1 NZ_CP017251:310365-310724 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT66323 NZ_CP017251:310365-310724 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References