Detailed information of TA system TA066321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 44086..44645
• Replicon: plasmid pO157
• Accession: NZ_CP017250
• Organism: Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: BHW76_RS28125
• Protein ID: WP_000421248.1
• Coordinates: 44086..44361 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: BHW76_RS28130
• Protein ID: WP_001178089.1
• Coordinates: 44361..44645 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHW76_RS28090	39439..39828	-	390	WP_001172748.1	cytochrome b562 family protein	-
BHW76_RS28095	39872..42082	-	2211	WP_000592771.1	catalase/peroxidase KatP	-
BHW76_RS28100	42259..42444	+	186	WP_001302179.1	hypothetical protein	-
BHW76_RS28105	42444..42860	+	417	Protein_35	IS91 family transposase	-
BHW76_RS30855	42860..43426	+	567	Protein_36	IS3-like element IS600 family transposase	-
BHW76_RS28115	43470..43685	+	216	Protein_37	transposase	-
BHW76_RS28120	43785..43991	-	207	WP_001105064.1	hypothetical protein	-
BHW76_RS28125	44086..44361	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BHW76_RS28130	44361..44645	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
BHW76_RS28135	45558..46415	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
BHW76_RS28140	46408..46482	-	75	WP_001370046.1	RepA leader peptide Tap	-
BHW76_RS28145	46718..46972	-	255	WP_000083831.1	replication regulatory protein RepA	-
BHW76_RS28150	47212..47550	-	339	WP_000766796.1	hypothetical protein	-
BHW76_RS28155	47588..47797	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
BHW76_RS28160	47843..48304	-	462	WP_001233853.1	thermonuclease family protein	-
BHW76_RS28165	48549..48761	-	213	WP_001302189.1	ANR family transcriptional regulator	-
BHW76_RS28170	48894..49454	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB	1..92690	92690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T66321 WP_000421248.1 NZ_CP017250:c44361-44086 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T66321 NZ_CP017250:c44361-44086 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT66321 WP_001178089.1 NZ_CP017250:c44645-44361 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT66321 NZ_CP017250:c44645-44361 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8