Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4791007..4791842 Replicon chromosome
Accession NZ_CP017249
Organism Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)


Gene name yeeV Uniprot ID B1LDL9
Locus tag BHW76_RS24630 Protein ID WP_001094444.1
Coordinates 4791007..4791384 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7LDN9
Locus tag BHW76_RS24635 Protein ID WP_001285607.1
Coordinates 4791474..4791842 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW76_RS24600 4787156..4787398 + 243 Protein_4669 N-acetylneuraminic acid mutarotase -
BHW76_RS24605 4787472..4788323 + 852 WP_000168589.1 HNH endonuclease -
BHW76_RS24610 4788521..4788708 - 188 Protein_4671 transposase -
BHW76_RS24615 4788783..4789610 - 828 WP_001301682.1 integrase arm-type DNA-binding domain-containing protein -
BHW76_RS32290 4790074..4790220 - 147 WP_001290178.1 hypothetical protein -
BHW76_RS24620 4790305..4790502 - 198 WP_001327226.1 DUF957 domain-containing protein -
BHW76_RS24625 4790522..4791010 - 489 WP_000761685.1 hypothetical protein -
BHW76_RS24630 4791007..4791384 - 378 WP_001094444.1 TA system toxin CbtA family protein Toxin
BHW76_RS24635 4791474..4791842 - 369 WP_001285607.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BHW76_RS24640 4791922..4792143 - 222 WP_000692350.1 DUF987 domain-containing protein -
BHW76_RS24645 4792230..4792706 - 477 WP_001186780.1 RadC family protein -
BHW76_RS24650 4792722..4793207 - 486 WP_000213716.1 antirestriction protein -
BHW76_RS24655 4793299..4794117 - 819 WP_001175175.1 DUF945 domain-containing protein -
BHW76_RS24660 4794207..4794416 - 210 WP_032150870.1 DUF905 family protein -
BHW76_RS24665 4794446..4795123 - 678 WP_001097301.1 hypothetical protein -
BHW76_RS24670 4795271..4795951 - 681 WP_001282919.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4783458..4828741 45283


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14087.02 Da        Isoelectric Point: 7.3523

>T66318 WP_001094444.1 NZ_CP017249:c4791384-4791007 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T66318 NZ_CP017249:c4791384-4791007 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13689.53 Da        Isoelectric Point: 6.4669

>AT66318 WP_001285607.1 NZ_CP017249:c4791842-4791474 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT66318 NZ_CP017249:c4791842-4791474 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B1LDL9

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0JLU8

References