Detailed information of TA system TA066315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4593337..4593887
• Replicon: chromosome
• Accession: NZ_CP017249
• Organism: Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: BHW76_RS23705
• Protein ID: WP_001160975.1
• Coordinates: 4593337..4593651 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: BHW76_RS23710
• Protein ID: WP_000125566.1
• Coordinates: 4593654..4593887 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHW76_RS23680	4589012..4590298	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
BHW76_RS23685	4590298..4591287	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
BHW76_RS23690	4591284..4592105	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
BHW76_RS23695	4592108..4592485	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
BHW76_RS23700	4592492..4593340	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
BHW76_RS23705	4593337..4593651	-	315	WP_001160975.1	CcdB family protein	Toxin
BHW76_RS23710	4593654..4593887	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
BHW76_RS23715	4593973..4594452	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
BHW76_RS23720	4594644..4596506	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
BHW76_RS23725	4596499..4597029	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
BHW76_RS23730	4597137..4598468	-	1332	WP_001183177.1	MFS transporter	-
BHW76_RS23735	4598526..4598813	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T66315 WP_001160975.1 NZ_CP017249:c4593651-4593337 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T66315 NZ_CP017249:c4593651-4593337 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT66315 WP_000125566.1 NZ_CP017249:c4593887-4593654 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT66315 NZ_CP017249:c4593887-4593654 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9