Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2755399..2756037 Replicon chromosome
Accession NZ_CP017249
Organism Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BHW76_RS14420 Protein ID WP_000813794.1
Coordinates 2755861..2756037 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BHW76_RS14415 Protein ID WP_001270286.1
Coordinates 2755399..2755815 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW76_RS14395 2750551..2751492 - 942 WP_001251315.1 ABC transporter permease -
BHW76_RS14400 2751493..2752506 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
BHW76_RS14405 2752524..2753669 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
BHW76_RS14410 2753914..2755320 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
BHW76_RS14415 2755399..2755815 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BHW76_RS14420 2755861..2756037 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BHW76_RS14425 2756259..2756489 + 231 WP_000494244.1 YncJ family protein -
BHW76_RS14430 2756581..2758542 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BHW76_RS14435 2758615..2759151 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
BHW76_RS14440 2759204..2760415 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T66300 WP_000813794.1 NZ_CP017249:c2756037-2755861 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T66300 NZ_CP017249:c2756037-2755861 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT66300 WP_001270286.1 NZ_CP017249:c2755815-2755399 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT66300 NZ_CP017249:c2755815-2755399 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References