Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2223597..2224392 Replicon chromosome
Accession NZ_CP017249
Organism Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag BHW76_RS11355 Protein ID WP_000854914.1
Coordinates 2224018..2224392 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag BHW76_RS11350 Protein ID WP_001280954.1
Coordinates 2223597..2223971 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW76_RS11310 2218954..2219859 + 906 WP_000203541.1 chemotaxis protein -
BHW76_RS11315 2219856..2220924 + 1069 Protein_2148 patatin-like phospholipase family protein -
BHW76_RS11325 2221264..2222082 + 819 WP_001234682.1 DUF945 domain-containing protein -
BHW76_RS11330 2222173..2222658 + 486 WP_000214398.1 antirestriction protein -
BHW76_RS11335 2222674..2223150 + 477 WP_001186738.1 RadC family protein -
BHW76_RS11340 2223213..2223434 + 222 WP_001220314.1 DUF987 domain-containing protein -
BHW76_RS11350 2223597..2223971 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BHW76_RS11355 2224018..2224392 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
BHW76_RS11360 2224389..2224880 + 492 WP_000976853.1 hypothetical protein -
BHW76_RS11365 2224892..2225089 + 198 WP_000839282.1 DUF957 domain-containing protein -
BHW76_RS11370 2225174..2226016 + 843 WP_001280481.1 DUF4942 domain-containing protein -
BHW76_RS11380 2226487..2227425 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
BHW76_RS11385 2227480..2228217 + 738 WP_000283664.1 zinc-binding phosphatase -
BHW76_RS11390 2228241..2228795 + 555 WP_001001917.1 molecular chaperone YcdY -
BHW76_RS11395 2228897..2229388 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroB / csgG / csgF / csgE / csgD / csgB / csgA / csgC 2192872..2234956 42084


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T66299 WP_000854914.1 NZ_CP017249:2224018-2224392 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T66299 NZ_CP017249:2224018-2224392 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT66299 WP_001280954.1 NZ_CP017249:2223597-2223971 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT66299 NZ_CP017249:2223597-2223971 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References