Detailed information of TA system TA066288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2142839..2143506
• Replicon: plasmid pRsp8C3c
• Accession: NZ_CP017244
• Organism: Rhizobium etli 8C-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A4R3R649
• Locus tag: AM571_RS35045
• Protein ID: WP_074065470.1
• Coordinates: 2143063..2143506 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AM571_RS35040
• Protein ID: WP_132549736.1
• Coordinates: 2142839..2143066 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM571_RS35015	2139206..2139520	+	315	WP_074065468.1	hypothetical protein	-
AM571_RS35020	2140326..2140799	+	474	WP_074065469.1	universal stress protein	-
AM571_RS35025	2140847..2141353	+	507	Protein_1979	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
AM571_RS35030	2141471..2141859	-	389	Protein_1980	transposase	-
AM571_RS36015	2142022..2142380	+	359	Protein_1981	transposase	-
AM571_RS36020	2142403..2142719	+	317	Protein_1982	IS66 family insertion sequence element accessory protein TnpB	-
AM571_RS35040	2142839..2143066	+	228	WP_132549736.1	CopG family transcriptional regulator	Antitoxin
AM571_RS35045	2143063..2143506	+	444	WP_074065470.1	PIN domain-containing protein	Toxin
AM571_RS35050	2143626..2144651	-	1026	WP_074065471.1	LysR family transcriptional regulator	-
AM571_RS35055	2144760..2145767	+	1008	WP_081377289.1	SDR family oxidoreductase	-
AM571_RS35060	2145803..2146210	-	408	WP_132658962.1	NUDIX domain-containing protein	-
AM571_RS35065	2146452..2147903	+	1452	WP_074065473.1	inorganic triphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA / htpB	1..2253963	2253963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15732.01 Da
• Isoelectric Point: 6.9773

>T66288 WP_074065470.1 NZ_CP017244:2143063-2143506 [Rhizobium etli 8C-3]
VTFLLDVNVMIALIDPGHVAHDDAHEWFVAMGQTAWATCPITENGVIRIVGNPKYPNSPGSPSLVMEIVRKLRSLPGHSF
WPDDVSLVGSDDIAPTKILTSGQVTDTYLLALAKARGGQLATFDRKLSAAAVTRGNSALHLITTSRS

Nucleotide

• Length: 444 bp

>T66288 NZ_CP017244:2143063-2143506 [Rhizobium etli 8C-3]
GTGACTTTCCTGCTCGACGTCAATGTGATGATTGCCTTAATCGATCCGGGCCATGTAGCCCATGATGATGCGCACGAATG
GTTCGTGGCGATGGGTCAGACCGCGTGGGCTACCTGCCCCATTACCGAAAATGGTGTCATCCGGATTGTCGGCAACCCCA
AATATCCCAACTCTCCCGGTTCACCGTCACTCGTGATGGAGATCGTCCGCAAATTGCGGTCGCTCCCCGGTCACAGTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCGGATGATATCGCTCCCACGAAAATTCTAACGTCGGGACAGGTCACTGACAC
CTACCTGCTTGCGCTAGCCAAGGCGCGTGGTGGCCAGCTGGCGACATTCGATCGCAAGCTGTCAGCAGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCTGATCACCACGAGTAGATCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.41 Da
• Isoelectric Point: 9.1096

>AT66288 WP_132549736.1 NZ_CP017244:2142839-2143066 [Rhizobium etli 8C-3]
MRTTLAIDDDVLIAAKAMATQQRRSVGEVISELARRSLRRPPSSGERNGIPLLSARPDAPPVTLEIVNALRDELP

Nucleotide

• Length: 228 bp

>AT66288 NZ_CP017244:2142839-2143066 [Rhizobium etli 8C-3]
ATGAGAACGACCTTGGCAATTGACGACGATGTCCTGATCGCCGCAAAAGCGATGGCAACCCAGCAGCGCCGGAGCGTCGG
CGAGGTCATTTCTGAGCTGGCGAGACGTTCTCTGCGCCGACCGCCCAGCAGCGGTGAGCGCAACGGTATTCCTCTCCTGT
CAGCTCGGCCGGATGCGCCGCCAGTGACGCTCGAAATCGTGAACGCCTTGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A4R3R649