Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2074051..2074724 Replicon plasmid pRsp8C3c
Accession NZ_CP017244
Organism Rhizobium etli 8C-3

Toxin (Protein)


Gene name vapC Uniprot ID A0A4V2VFR7
Locus tag AM571_RS34680 Protein ID WP_074065419.1
Coordinates 2074320..2074724 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R3R886
Locus tag AM571_RS34675 Protein ID WP_074065418.1
Coordinates 2074051..2074323 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM571_RS34660 2071897..2072484 - 588 WP_074065417.1 RES family NAD+ phosphorylase -
AM571_RS34665 2072481..2073101 - 621 WP_074065751.1 hypothetical protein -
AM571_RS34670 2073400..2073615 + 216 WP_081377282.1 DUF2092 domain-containing protein -
AM571_RS34675 2074051..2074323 + 273 WP_074065418.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM571_RS34680 2074320..2074724 + 405 WP_074065419.1 type II toxin-antitoxin system VapC family toxin Toxin
AM571_RS36000 2074815..2075018 - 204 Protein_1915 transposase domain-containing protein -
AM571_RS36800 2075015..2075193 - 179 Protein_1916 IS3 family transposase -
AM571_RS34695 2075359..2077221 - 1863 WP_074065752.1 adenylate/guanylate cyclase domain-containing protein -
AM571_RS34710 2078807..2079074 - 268 Protein_1918 hypothetical protein -
AM571_RS34715 2079218..2079673 - 456 WP_074065420.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA / htpB 1..2253963 2253963


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(16-53)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14800.19 Da        Isoelectric Point: 4.7581

>T66287 WP_074065419.1 NZ_CP017244:2074320-2074724 [Rhizobium etli 8C-3]
MNGYLLDTNIISDIIRNPFGPAARRIEEIDPKEICTSIIVAAELRYGCAKKGSARLLAKVESVLETLPILPMDIPADIKY
GGIRAELEAVGQSIGLNDLLIAAHACVLDLTLVTDNTREFQRIRGLNLENWLER

Download         Length: 405 bp

>T66287 NZ_CP017244:2074320-2074724 [Rhizobium etli 8C-3]
TTGAACGGGTACTTGCTTGATACAAACATCATCAGCGACATCATACGCAATCCCTTCGGACCGGCGGCTCGCCGCATCGA
GGAGATCGATCCCAAAGAGATTTGCACCAGTATCATCGTCGCAGCTGAGTTGCGATACGGTTGCGCAAAAAAGGGATCAG
CCAGGCTACTGGCCAAGGTCGAAAGCGTGCTCGAAACGCTTCCCATACTGCCCATGGATATCCCCGCCGATATCAAGTAC
GGCGGCATCCGAGCGGAGTTGGAAGCGGTGGGGCAGAGCATTGGCCTCAACGATCTACTGATCGCCGCGCATGCCTGTGT
GCTAGACCTGACACTTGTCACGGACAATACGCGCGAGTTCCAGCGCATTCGCGGCCTCAATCTGGAGAACTGGCTTGAGA
GATAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10292.82 Da        Isoelectric Point: 4.6909

>AT66287 WP_074065418.1 NZ_CP017244:2074051-2074323 [Rhizobium etli 8C-3]
MPISLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVMIHREGDKLIIEPVTGPSNFAELIAEWKREPPLGPEDQFPDIE
DMIAKPENIF

Download         Length: 273 bp

>AT66287 NZ_CP017244:2074051-2074323 [Rhizobium etli 8C-3]
ATGCCTATTTCATTGCCGTCATCAAGACCGAAAGAAGTGAAGCTGTTCCGCAACAACCGCAGCCAGGCGGTGCGCATCCC
TGCGGAATTTGAATTGCCAGGGGATCGCGTGATGATCCACCGCGAGGGGGATAAGCTCATCATCGAGCCTGTAACCGGGC
CGTCCAATTTCGCGGAGCTTATTGCCGAGTGGAAGAGGGAGCCTCCGCTCGGGCCTGAAGACCAGTTCCCGGATATTGAG
GATATGATTGCAAAGCCGGAGAACATCTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4V2VFR7

Antitoxin

Source ID Structure
AlphaFold DB A0A4R3R886

References