Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 185267..185937 Replicon plasmid pRsp8C3c
Accession NZ_CP017244
Organism Rhizobium etli 8C-3

Toxin (Protein)


Gene name vapC Uniprot ID A0A4R3RXL7
Locus tag AM571_RS25710 Protein ID WP_074063880.1
Coordinates 185518..185937 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R3RZE4
Locus tag AM571_RS25705 Protein ID WP_074063879.1
Coordinates 185267..185521 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM571_RS25690 181546..182130 - 585 WP_196776380.1 GNAT family N-acetyltransferase -
AM571_RS25695 182306..182737 + 432 WP_074063877.1 GNAT family N-acetyltransferase -
AM571_RS25700 183049..184815 - 1767 WP_074063878.1 adenylate/guanylate cyclase domain-containing protein -
AM571_RS25705 185267..185521 + 255 WP_074063879.1 plasmid stabilization protein Antitoxin
AM571_RS25710 185518..185937 + 420 WP_074063880.1 type II toxin-antitoxin system VapC family toxin Toxin
AM571_RS25715 186220..186789 - 570 WP_074063881.1 nucleoside 2-deoxyribosyltransferase -
AM571_RS25720 186966..187157 + 192 Protein_176 Rrf2 family transcriptional regulator -
AM571_RS25725 187504..189444 + 1941 WP_074063882.1 DUF3604 domain-containing protein -
AM571_RS25730 189531..190370 + 840 WP_074063883.1 peptidyl-prolyl cis-trans isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA / htpB 1..2253963 2253963


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14943.24 Da        Isoelectric Point: 7.0136

>T66286 WP_074063880.1 NZ_CP017244:185518-185937 [Rhizobium etli 8C-3]
MILLDTNVISEPWKPAPNGAVVAWMDAQVIETLFLSAITIAELRFGIAAMPSGKRQTVLRDRLEGEVLPHFAERVLPFNS
ATSQFYSELMVRARLSGKAVGKADGYIAATAAANGLAVATRDTSPFEAAGLKVINPWSQ

Download         Length: 420 bp

>T66286 NZ_CP017244:185518-185937 [Rhizobium etli 8C-3]
ATGATCTTGTTGGACACCAACGTGATATCGGAGCCATGGAAACCGGCTCCCAACGGGGCAGTGGTAGCATGGATGGATGC
CCAAGTCATCGAGACGTTGTTCCTTTCCGCGATAACAATTGCCGAACTTCGCTTCGGGATTGCCGCCATGCCTTCAGGCA
AACGGCAAACAGTCCTTCGCGATCGCCTGGAGGGCGAAGTGCTACCTCACTTCGCAGAACGCGTCCTACCCTTTAATTCA
GCCACCTCCCAATTCTACTCGGAACTCATGGTGCGCGCTCGCTTGTCCGGGAAGGCGGTCGGCAAGGCGGATGGATATAT
TGCAGCCACAGCAGCGGCGAATGGCCTCGCGGTCGCAACGCGCGACACAAGCCCCTTCGAGGCAGCCGGATTGAAAGTGA
TCAATCCATGGTCCCAATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9285.40 Da        Isoelectric Point: 5.6344

>AT66286 WP_074063879.1 NZ_CP017244:185267-185521 [Rhizobium etli 8C-3]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPSERVKLGSLLAAIGRDAELSNDDVEALQQNRDKAPAEP
MTFE

Download         Length: 255 bp

>AT66286 NZ_CP017244:185267-185521 [Rhizobium etli 8C-3]
ATGCCGGCGGTCACCATTCGTAATCTTTCTGATGAAACGCATCGCGCGCTGCGCGTGCGAGCGGCTCATCACGGCCGTAG
TACGGAGGCCGAAATCCGTGACATTATTGAGGCCGCCGTTCGTCCATCTGAACGTGTGAAACTTGGCTCTTTACTCGCGG
CCATAGGTCGGGACGCGGAGCTATCCAATGACGATGTTGAAGCCCTGCAGCAAAATCGCGATAAGGCGCCAGCCGAACCG
ATGACGTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R3RXL7

Antitoxin

Source ID Structure
AlphaFold DB A0A4R3RZE4

References