Detailed information of TA system TA066283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2609015..2609580
• Replicon: chromosome
• Accession: NZ_CP017239
• Organism: Burkholderia cenocepacia strain CR318

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BHQ31_RS28720
• Protein ID: WP_011546880.1
• Coordinates: 2609209..2609580 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1FGI9
• Locus tag: BHQ31_RS28715
• Protein ID: WP_006752328.1
• Coordinates: 2609015..2609212 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHQ31_RS28690	2604318..2604773	-	456	WP_006480483.1	RbsD/FucU family protein	-
BHQ31_RS28695	2604852..2605244	-	393	WP_077177808.1	nuclear transport factor 2 family protein	-
BHQ31_RS28700	2605463..2607016	-	1554	WP_011546882.1	galactarate dehydratase	-
BHQ31_RS28705	2607246..2608160	+	915	WP_011546881.1	5-dehydro-4-deoxyglucarate dehydratase	-
BHQ31_RS28710	2608275..2608829	-	555	WP_006480480.1	hypothetical protein	-
BHQ31_RS28715	2609015..2609212	+	198	WP_006752328.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BHQ31_RS28720	2609209..2609580	+	372	WP_011546880.1	PIN domain-containing protein	Toxin
BHQ31_RS28725	2609708..2611285	-	1578	WP_011546879.1	aldehyde dehydrogenase (NADP(+))	-
BHQ31_RS28730	2611572..2612924	+	1353	WP_198174621.1	MFS transporter	-
BHQ31_RS28735	2612991..2613716	-	726	WP_078041702.1	site-2 protease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13559.67 Da
• Isoelectric Point: 6.2156

>T66283 WP_011546880.1 NZ_CP017239:2609209-2609580 [Burkholderia cenocepacia]
MILVDTSVWIDHLRAGDATLTALLEAERVLIHPFIVGELALGSMRHRETVLDALRDLPAATLATDDEVQRMIDAVPLHGL
GIGYVDAHLLASARLTSDSRLWTRDRRLLAAAERLQLVARPAH

Nucleotide

• Length: 372 bp

>T66283 NZ_CP017239:2609209-2609580 [Burkholderia cenocepacia]
ATGATCCTTGTCGACACATCCGTCTGGATCGATCACTTGCGAGCCGGCGACGCGACGCTCACCGCCTTGCTGGAAGCGGA
GCGAGTATTGATCCATCCGTTCATCGTCGGCGAGCTTGCGCTCGGCAGCATGCGCCATCGCGAGACCGTGCTGGATGCGC
TGCGCGACCTTCCAGCTGCAACCCTTGCCACCGACGACGAAGTCCAGCGCATGATCGATGCCGTACCGCTTCACGGGCTG
GGCATCGGGTACGTCGATGCCCACCTGCTTGCGTCGGCCAGACTGACCAGCGACAGCAGGCTATGGACGCGCGACCGGCG
CCTGCTGGCGGCAGCGGAGCGCCTGCAACTCGTCGCCCGCCCCGCTCACTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7054.10 Da
• Isoelectric Point: 10.6826

>AT66283 WP_006752328.1 NZ_CP017239:2609015-2609212 [Burkholderia cenocepacia]
MRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSEPA

Nucleotide

• Length: 198 bp

>AT66283 NZ_CP017239:2609015-2609212 [Burkholderia cenocepacia]
ATGCGCACCACCTTGTCCCTCGACGACGCACTGCTCGCCAAAGCGCAGCAATTGACCGGCGTCACCGAAAAATCGGCGCT
CGTGAGGGAAGCGCTGCGCGCATTGATCGCCCGCGAAAGTGCGCGACGACTCGCCCGCCTCGGCGGCACCGAGCCTGATC
TTGAATCGGTCCCACGCCGCCCGTCGGAGCCCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KMB8