Detailed information of TA system TA066282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2128623..2129849
• Replicon: chromosome
• Accession: NZ_CP017239
• Organism: Burkholderia cenocepacia strain CR318

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B1K807
• Locus tag: BHQ31_RS26605
• Protein ID: WP_011547213.1
• Coordinates: 2129268..2129849 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BHQ31_RS26600
• Protein ID: WP_011547214.1
• Coordinates: 2128623..2129189 (+)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHQ31_RS26575	2123689..2124294	-	606	WP_102961848.1	hypothetical protein	-
BHQ31_RS26580	2124555..2125208	+	654	WP_011547217.1	cysteine hydrolase	-
BHQ31_RS26585	2125234..2125965	-	732	WP_006498851.1	ribonuclease T2	-
BHQ31_RS26590	2126093..2126671	-	579	WP_012339630.1	hypothetical protein	-
BHQ31_RS26595	2126771..2128174	-	1404	WP_012339629.1	hypothetical protein	-
BHQ31_RS26600	2128623..2129189	+	567	WP_011547214.1	helix-turn-helix domain-containing protein	Antitoxin
BHQ31_RS26605	2129268..2129849	+	582	WP_011547213.1	HD domain-containing protein	Toxin
BHQ31_RS26615	2130258..2131451	+	1194	WP_011547212.1	efflux RND transporter periplasmic adaptor subunit	-
BHQ31_RS26620	2131454..2133499	+	2046	WP_012339627.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-172)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21317.94 Da
• Isoelectric Point: 5.1207

>T66282 WP_011547213.1 NZ_CP017239:2129268-2129849 [Burkholderia cenocepacia]
MNPDTIHARLAFLREAERLKDVLRSSHTSAGRPESTAEHSWRLCLMALVFTDALPGVDALKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTASLDRAVRDEIVALWDEYEAAASPEARAAKALDKLETIVQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFRAIREIVDADTQRKIDAGGERA

Nucleotide

• Length: 582 bp

>T66282 NZ_CP017239:2129268-2129849 [Burkholderia cenocepacia]
ATGAACCCCGATACGATCCACGCGCGACTCGCGTTCCTGCGCGAAGCCGAACGCCTGAAGGACGTGCTGCGCAGCAGCCA
CACGTCGGCCGGCCGCCCGGAAAGCACGGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCACCGACGCAC
TGCCCGGCGTCGACGCGCTGAAGCTGCTGAAACTGTGCGTCGTCCACGACCTCGGCGAGGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCATCCCGACAAGAGCGCACATGAACGCGACGACCTGCTGACGCTGACCGCGTCGCTAGACCG
CGCGGTGCGCGACGAGATCGTCGCGCTGTGGGACGAATACGAGGCGGCCGCCTCACCCGAAGCCCGCGCGGCGAAGGCGC
TCGACAAGCTCGAAACGATCGTGCAGCACAACCAGGGCAGCAATCCGCCCGATTTCGACTATGCGTTCAATCTCGGCTAC
GGCCGGCGCTATACCGATGCTGCGCCGCTGTTCCGCGCGATCCGCGAAATCGTCGACGCCGATACGCAGCGCAAGATCGA
CGCGGGTGGGGAGCGCGCATAG

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20565.46 Da
• Isoelectric Point: 10.7076

>AT66282 WP_011547214.1 NZ_CP017239:2128623-2129189 [Burkholderia cenocepacia]
MPHPPLHPSSLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
SAGRMHLVCDLTHRLLASSPARAAEFSQPLSTLLGTSLWRYATPEIVRMEAALDPFGWHDRAGPPSVEFDTGANASRVVP
IRGSRCRWTRMTLSDSSAVRLVETLDAH

Nucleotide

• Length: 567 bp

>AT66282 NZ_CP017239:2128623-2129189 [Burkholderia cenocepacia]
ATGCCCCATCCGCCGCTTCATCCATCCTCGCTCGGCCCGCAACTCAAGCGCTGGCGCGCGCTGCACCGCGTGAAGCAAAG
CCATGCAGCCGAACTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCCTCCAGCAGATGTCGCCCGCCG
AGCGCGCGACGGCCGAACGGCTGCTCGCGGCGCGCCTCGACTCGGCGGGCGACCACGCACTCGCGCGGCTGATCGCCGGC
AGCGCGGGCCGCATGCATCTCGTGTGCGACCTCACGCACCGCCTGCTGGCCAGTTCCCCAGCACGCGCGGCCGAGTTCTC
GCAACCGCTTTCGACGTTGCTCGGCACGTCGCTGTGGCGCTACGCGACACCGGAGATCGTCCGCATGGAAGCCGCGCTCG
ACCCGTTCGGCTGGCACGACCGCGCGGGGCCGCCGAGCGTCGAGTTCGACACCGGCGCGAATGCGTCGCGCGTCGTGCCG
ATTCGCGGCAGCCGATGCCGGTGGACACGGATGACACTGTCGGACAGCTCGGCCGTGCGGCTCGTGGAGACGCTCGACGC
ACACTGA

Structures

Toxin

• AlphaFold DB: B1K807