Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1144567..1145144 Replicon chromosome
Accession NZ_CP017239
Organism Burkholderia cenocepacia strain CR318

Toxin (Protein)


Gene name vapC Uniprot ID A0A3R9B004
Locus tag BHQ31_RS22155 Protein ID WP_011547972.1
Coordinates 1144567..1144932 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B1K204
Locus tag BHQ31_RS22160 Protein ID WP_011547971.1
Coordinates 1144926..1145144 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHQ31_RS22135 1140002..1140901 + 900 WP_078041461.1 lipid A hydroxylase LpxO -
BHQ31_RS22140 1141058..1141891 + 834 WP_011547975.1 hypothetical protein -
BHQ31_RS22145 1141987..1143588 - 1602 WP_078041462.1 alkyl hydroperoxide reductase subunit F -
BHQ31_RS22150 1143707..1144270 - 564 WP_011547973.1 peroxiredoxin -
BHQ31_RS22155 1144567..1144932 - 366 WP_011547972.1 type II toxin-antitoxin system VapC family toxin Toxin
BHQ31_RS22160 1144926..1145144 - 219 WP_011547971.1 hypothetical protein Antitoxin
BHQ31_RS22165 1145236..1146132 - 897 WP_011547970.1 LysR family transcriptional regulator -
BHQ31_RS22170 1146313..1147479 + 1167 WP_011547969.1 branched-chain amino acid ABC transporter substrate-binding protein -
BHQ31_RS22175 1147521..1148045 + 525 WP_011547968.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
BHQ31_RS22180 1148038..1149294 + 1257 WP_011547967.1 Zn-dependent hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13396.44 Da        Isoelectric Point: 8.4487

>T66278 WP_011547972.1 NZ_CP017239:c1144932-1144567 [Burkholderia cenocepacia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISMISWMEVLVGASASDEAPIRAWLSSFNVIALDSTVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPASEPGVRVPYQI

Download         Length: 366 bp

>T66278 NZ_CP017239:c1144932-1144567 [Burkholderia cenocepacia]
ATGGTAAAAGCCCTTTTCGACACCAACATCCTGATCGATTATCTGGGCGGCGTGGAATCCGCGCGTGCGGAGCTAGGCCG
CTACGACTATCGCGCAATCAGCATGATCTCGTGGATGGAAGTCCTCGTCGGGGCGTCCGCAAGTGACGAGGCCCCCATCC
GTGCATGGCTCTCGTCGTTCAATGTCATCGCGCTCGACAGCACGGTGGCGAATCGCGCCGTGACGATTCGCAAGGAACGC
CGCATACGTCTGCCCGACGCAATCGTGTGGGCGTCAGCACAAGTGAACGGGCTGTTGCTCGTATCGCGCAACACGAAGGA
CTTTCCCGCCTCCGAGCCCGGCGTTCGCGTGCCATATCAGATCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8292.38 Da        Isoelectric Point: 4.5560

>AT66278 WP_011547971.1 NZ_CP017239:c1145144-1144926 [Burkholderia cenocepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALNKHRLADDVFGLWKDRTVDGLAYQEELRSEW

Download         Length: 219 bp

>AT66278 NZ_CP017239:c1145144-1144926 [Burkholderia cenocepacia]
ATGAGCCGGATTCTGGTCGATCTGTCGGATGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACACCGTCCCCG
CGCCGCCATCATTCGTGACGCGATCGACGCCTACATTGCCCTGAACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTAT
GGAAAGATCGCACGGTCGACGGGCTCGCCTATCAAGAGGAATTGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9B004

Antitoxin

Source ID Structure
AlphaFold DB B1K204

References