Detailed information of TA system TA066276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2251884..2252490
• Replicon: chromosome
• Accession: NZ_CP017237
• Organism: Moorella thermoacetica strain DSM 103284

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1D7XDY8
• Locus tag: MTJW_RS11235
• Protein ID: WP_011393652.1
• Coordinates: 2251884..2252234 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MTJW_RS11240
• Protein ID: WP_036371414.1
• Coordinates: 2252236..2252490 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTJW_RS11200	2247490..2247975	-	486	WP_036371410.1	ribosomal protein S18-alanine N-acetyltransferase	-
MTJW_RS11205	2247993..2248679	-	687	WP_011393646.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
MTJW_RS11210	2248660..2249127	-	468	WP_071542274.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
MTJW_RS11215	2249288..2249863	-	576	WP_071542546.1	uracil-DNA glycosylase	-
MTJW_RS11220	2249918..2250550	-	633	WP_025773383.1	thiamine phosphate synthase	-
MTJW_RS11225	2250631..2251020	-	390	WP_025773384.1	response regulator	-
MTJW_RS11230	2251169..2251870	-	702	WP_071542275.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
MTJW_RS11235	2251884..2252234	-	351	WP_011393652.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MTJW_RS11240	2252236..2252490	-	255	WP_036371414.1	ribbon-helix-helix protein, CopG family	Antitoxin
MTJW_RS11245	2252618..2253739	-	1122	WP_071542276.1	alanine racemase	-
MTJW_RS11250	2253711..2255303	-	1593	WP_071542277.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
MTJW_RS11255	2255624..2256055	-	432	WP_071542278.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12684.71 Da
• Isoelectric Point: 7.2034

>T66276 WP_011393652.1 NZ_CP017237:c2252234-2251884 [Moorella thermoacetica]
MLVRRGDVFYAHLNPVVGSEQGGTRPVLIIQNDIGNQYSPTTIVAAITSQIAKAKLPTHVEISAAKSGLERDSVILLEQI
RTIDKSRLKQKVAVLDDEILEKVNRALEISLGLVEV

Nucleotide

• Length: 351 bp

>T66276 NZ_CP017237:c2252234-2251884 [Moorella thermoacetica]
ATGCTGGTACGGCGCGGGGATGTCTTTTACGCCCATCTCAATCCGGTGGTCGGTTCCGAGCAAGGCGGAACCCGCCCGGT
ACTTATCATTCAAAATGACATTGGCAACCAGTATAGCCCCACTACTATTGTGGCCGCTATTACTTCCCAGATTGCCAAGG
CCAAACTGCCCACCCATGTGGAAATCAGCGCTGCCAAGAGCGGCCTGGAGCGCGATTCTGTTATCCTGCTGGAACAGATC
CGCACCATAGACAAGAGCCGCCTCAAACAAAAAGTGGCCGTCCTGGATGATGAGATCCTGGAAAAGGTGAACCGGGCCCT
GGAAATAAGCCTGGGACTGGTGGAAGTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9983.45 Da
• Isoelectric Point: 6.3653

>AT66276 WP_036371414.1 NZ_CP017237:c2252490-2252236 [Moorella thermoacetica]
MISLPESLLAEVDGLANLEKRNRSEFIREAMKLYITERKRRNIREQMKRGYQEMAPINLALAVENYDVENEVQRYYDKSL
AECK

Nucleotide

• Length: 255 bp

>AT66276 NZ_CP017237:c2252490-2252236 [Moorella thermoacetica]
ATGATCAGCTTGCCGGAGAGCCTCCTGGCCGAGGTTGACGGCCTGGCGAACCTTGAAAAGCGTAACCGCAGCGAGTTTAT
CCGGGAAGCTATGAAACTATATATTACCGAGCGTAAAAGGCGCAATATCCGGGAACAAATGAAACGGGGATACCAGGAAA
TGGCACCTATCAATTTAGCCCTTGCAGTGGAAAACTATGATGTAGAGAATGAAGTACAAAGATACTACGATAAAAGCCTG
GCGGAGTGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A1D7XDY8