Detailed information of TA system TA066275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 961549..962123
• Replicon: chromosome
• Accession: NZ_CP017237
• Organism: Moorella thermoacetica strain DSM 103284

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MTJW_RS04810
• Protein ID: WP_071541482.1
• Coordinates: 961776..962123 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1D7X9U1
• Locus tag: MTJW_RS04805
• Protein ID: WP_053094734.1
• Coordinates: 961549..961782 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTJW_RS04775	956984..957670	-	687	WP_011392530.1	manganese catalase family protein	-
MTJW_RS04785	958253..958486	+	234	WP_071541478.1	hypothetical protein	-
MTJW_RS04790	958470..958733	+	264	WP_071541479.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MTJW_RS13310	959805..960425	+	621	WP_196768306.1	YdcF family protein	-
MTJW_RS13315	960427..961209	+	783	WP_071541481.1	hypothetical protein	-
MTJW_RS04805	961549..961782	+	234	WP_053094734.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MTJW_RS04810	961776..962123	+	348	WP_071541482.1	endoribonuclease MazF	Toxin
MTJW_RS04815	962804..963016	+	213	WP_196768307.1	copper amine oxidase N-terminal domain-containing protein	-
MTJW_RS13320	963032..963373	+	342	WP_196768308.1	MBL fold metallo-hydrolase	-
MTJW_RS13325	963324..963779	+	456	WP_196768309.1	hypothetical protein	-
MTJW_RS04830	963855..964343	-	489	Protein_944	IS110 family transposase	-
MTJW_RS04840	964384..965561	+	1178	WP_155767725.1	IS3 family transposase	-
MTJW_RS04845	965881..966465	+	585	WP_081361080.1	glycerol-3-phosphate responsive antiterminator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-47)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12734.87 Da
• Isoelectric Point: 8.4951

>T66275 WP_071541482.1 NZ_CP017237:961776-962123 [Moorella thermoacetica]
MVNEMETYIPERGDIVWLQFDPRAGHEQARRRPALVISPQLYNGKVGLALFCPVTTKTKGYPFEVEIPAGLKITGVILAD
QVKSLDWRAREAQFACKVPAGVVAEVQAKVQVLIS

Nucleotide

• Length: 348 bp

>T66275 NZ_CP017237:961776-962123 [Moorella thermoacetica]
ATGGTAAACGAGATGGAAACCTATATTCCCGAACGTGGTGATATAGTCTGGCTCCAGTTTGATCCCCGGGCAGGACATGA
GCAAGCTCGCAGGCGGCCGGCGCTGGTAATTTCACCGCAACTTTATAACGGCAAAGTGGGACTGGCCCTTTTTTGCCCAG
TTACTACAAAGACGAAGGGTTATCCCTTTGAAGTAGAGATACCGGCTGGTTTAAAGATTACCGGAGTGATCCTGGCAGAC
CAGGTAAAAAGCCTTGACTGGCGGGCAAGGGAGGCGCAATTTGCCTGTAAAGTCCCGGCAGGGGTGGTAGCAGAAGTCCA
GGCTAAAGTTCAGGTGTTGATTTCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8798.14 Da
• Isoelectric Point: 7.2029

>AT66275 WP_053094734.1 NZ_CP017237:961549-961782 [Moorella thermoacetica]
MQPHVQKWGNSLGIRIPLSLAQKIGLREGTPVDLQVDEDAIIIRRKQYSLEQMLARVKPENIHSEIDTGPQVGREIW

Nucleotide

• Length: 234 bp

>AT66275 NZ_CP017237:961549-961782 [Moorella thermoacetica]
ATGCAACCTCATGTGCAAAAGTGGGGCAATAGTTTAGGGATACGTATCCCTTTGTCGCTGGCCCAAAAAATCGGCCTTAG
AGAAGGCACACCGGTTGATTTACAGGTAGATGAGGACGCGATAATTATCCGCCGCAAACAATACAGCCTGGAACAAATGT
TAGCCCGGGTTAAACCTGAAAATATTCATAGTGAAATAGATACCGGGCCTCAGGTTGGGCGGGAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D7X9U1