Detailed information of TA system TA066274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3323147..3323681
• Replicon: chromosome
• Accession: NZ_CP017236
• Organism: Yersinia ruckeri strain QMA0440 isolate 14/0165-5k

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QMA0440_RS15585
• Protein ID: WP_038245762.1
• Coordinates: 3323147..3323437 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A1JJ37
• Locus tag: QMA0440_RS15590
• Protein ID: WP_005175264.1
• Coordinates: 3323427..3323681 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMA0440_RS15540	3318422..3318652	-	231	WP_080717349.1	DUF4942 domain-containing protein	-
QMA0440_RS18270	3318802..3318930	+	129	Protein_2968	helix-turn-helix domain-containing protein	-
QMA0440_RS15545	3318954..3319043	+	90	Protein_2969	transposase	-
QMA0440_RS15550	3319072..3319362	-	291	Protein_2970	transposase	-
QMA0440_RS15555	3319367..3319765	+	399	Protein_2971	DDE-type integrase/transposase/recombinase	-
QMA0440_RS15560	3319784..3320050	-	267	Protein_2972	hypothetical protein	-
QMA0440_RS15565	3320071..3321138	-	1068	WP_038245756.1	macro domain-containing protein	-
QMA0440_RS15570	3321135..3321791	-	657	WP_038245758.1	DUF4433 domain-containing protein	-
QMA0440_RS15575	3321794..3322369	-	576	WP_038245760.1	DUF4433 domain-containing protein	-
QMA0440_RS15580	3322416..3322883	-	468	WP_051847072.1	restriction endonuclease subunit S	-
QMA0440_RS15585	3323147..3323437	-	291	WP_038245762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMA0440_RS15590	3323427..3323681	-	255	WP_005175264.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
QMA0440_RS15595	3323855..3324307	-	453	Protein_2979	site-specific integrase	-
QMA0440_RS15600	3324355..3324663	-	309	WP_038245765.1	hypothetical protein	-
QMA0440_RS15605	3324739..3325650	-	912	WP_080717354.1	toll/interleukin-1 receptor domain-containing protein	-
QMA0440_RS15610	3325690..3326840	+	1151	WP_087931031.1	IS3 family transposase	-
QMA0440_RS15615	3326899..3327267	+	369	WP_071704745.1	DUF1173 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3320071..3326840	6769
	-	flank	IS/Tn	-	-	3319072..3319320	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11494.42 Da
• Isoelectric Point: 9.9816

>T66274 WP_038245762.1 NZ_CP017236:c3323437-3323147 [Yersinia ruckeri]
MTFNIDFDERALKEWHKLDKTIREQFKKKLRKLQENPYIESARLHGDLAGCFKIKLRASGFRLIYQVIDEEIVIWIVAVG
KREDEKAYDLAKKRIQ

Nucleotide

• Length: 291 bp

>T66274 NZ_CP017236:c3323437-3323147 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAGTCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTGGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATCTTGCCAAGAAGCGAATCCAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9680.07 Da
• Isoelectric Point: 4.8283

>AT66274 WP_005175264.1 NZ_CP017236:c3323681-3323427 [Yersinia ruckeri]
MSTIQIRVDDELKKSAYQAFEKLNLSPSDALRLFLRYVAENEKLPFSEVSVMVSNQDEDADILAVVRERLKNPAKRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT66274 NZ_CP017236:c3323681-3323427 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA

Structures

Antitoxin

• AlphaFold DB: A1JJ37