Detailed information of TA system TA066261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2352381..2353359
• Replicon: chromosome
• Accession: NZ_CP017234
• Organism: Bacillus cereus strain FORC_048

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: FORC48_RS12410
• Protein ID: WP_000624977.1
• Coordinates: 2352381..2353118 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: FORC48_RS12415
• Protein ID: WP_000237818.1
• Coordinates: 2353231..2353359 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC48_RS12385	2348024..2348806	+	783	WP_000381792.1	class I SAM-dependent methyltransferase	-
FORC48_RS12390	2348964..2350649	-	1686	WP_033695152.1	alpha-keto acid decarboxylase family protein	-
FORC48_RS12395	2350757..2351239	+	483	WP_000191886.1	MarR family winged helix-turn-helix transcriptional regulator	-
FORC48_RS12400	2351406..2352143	+	738	WP_000594136.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FORC48_RS12410	2352381..2353118	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
FORC48_RS12415	2353231..2353359	+	129	WP_000237818.1	hypothetical protein	Antitoxin
FORC48_RS12420	2353432..2353608	+	177	WP_000852629.1	stage II sporulation protein SB	-
FORC48_RS12425	2353627..2354016	-	390	WP_000713865.1	YxeA family protein	-
FORC48_RS12430	2354228..2355697	+	1470	WP_000287543.1	beta-Ala-His dipeptidase	-
FORC48_RS12435	2355943..2356752	+	810	WP_000664543.1	C39 family peptidase	-
FORC48_RS12440	2356778..2357380	+	603	WP_000517265.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T66261 WP_000624977.1 NZ_CP017234:2352381-2353118 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T66261 NZ_CP017234:2352381-2353118 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCAAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTGGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT66261 WP_000237818.1 NZ_CP017234:2353231-2353359 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT66261 NZ_CP017234:2353231-2353359 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAGGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5