Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240903..241545 Replicon chromosome
Accession NZ_CP017234
Organism Bacillus cereus strain FORC_048

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FORC48_RS01415 Protein ID WP_000635965.1
Coordinates 241195..241545 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FORC48_RS01410 Protein ID WP_000004570.1
Coordinates 240903..241190 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC48_RS01385 236220..237182 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
FORC48_RS01390 237175..237747 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FORC48_RS01395 237841..238200 + 360 WP_000583415.1 holo-ACP synthase -
FORC48_RS01400 238357..239307 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
FORC48_RS01405 239425..240594 + 1170 WP_000390615.1 alanine racemase -
FORC48_RS01410 240903..241190 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FORC48_RS01415 241195..241545 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FORC48_RS01420 241613..243781 + 2169 WP_044306890.1 RNA-binding transcriptional accessory protein -
FORC48_RS01425 243839..243955 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FORC48_RS01430 244151..244609 + 459 WP_044306889.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T66260 WP_000635965.1 NZ_CP017234:241195-241545 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T66260 NZ_CP017234:241195-241545 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT66260 WP_000004570.1 NZ_CP017234:240903-241190 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT66260 NZ_CP017234:240903-241190 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References